public void different_classes_with_same_id_value_should_not_be_equal()
{
var a = new SampleId("a");
var b = new AnotherSampleId("a");
Assert.IsFalse(a.Equals(b));
}
public override int GetHashCode()
{
int hash = 1;
if (SampleId != 0UL)
{
hash ^= SampleId.GetHashCode();
}
hash ^= data_.GetHashCode();
return(hash);
}
public void test_equality()
{
var a = new SampleId("a");
var b = new SampleId("a");
Assert.AreEqual(a, b);
Assert.IsTrue(a.Equals(b));
Assert.IsTrue(a.Equals(a));
Assert.IsTrue(a == b);
}
public void test_inequality()
{
var a = new SampleId("a");
var b = new SampleId("b");
Assert.AreNotEqual(a, null);
Assert.AreNotEqual(a, b);
Assert.IsFalse(a.Equals(b));
Assert.IsFalse(a.Equals(null));
Assert.IsFalse(a == b);
}
private static ReferencePloidy LoadReferencePloidy(IEnumerable <ReferencePloidyInterval> ploidyVcfIntervals,
bool useSymbolicAltAlleleInPloidyVcf = true)
{
var sampleId = new SampleId("sampleId");
var vcf = GetVcfAsString(sampleId, ploidyVcfIntervals, useSymbolicAltAlleleInPloidyVcf);
using (var textReader = new StringReader(vcf))
using (var reader = new GzipOrTextReader(textReader))
using (var vcfReader = new VcfReader(reader))
{
return(ReferencePloidy.Load(vcfReader, sampleId));
}
}
public SoundEffectEvent(
SampleId soundId,
byte volume,
byte restartProbability,
byte unk3,
ushort frequencyOverride,
SoundMode mode)
{
SoundId = soundId;
Volume = volume;
RestartProbability = restartProbability;
Unk3 = unk3;
FrequencyOverride = frequencyOverride;
Mode = mode;
}
public static bool IsSharedCnv(ISampleMap <Genotype> copyNumberGenotypes, ISampleMap <CanvasSegment> canvasSegments, ISampleMap <SampleMetrics> samplesInfo, List <SampleId> parentIDs,
SampleId probandId, int maximumCopyNumber)
{
var proband = copyNumberGenotypes[probandId];
var parent1 = copyNumberGenotypes[parentIDs.First()];
var parent2 = copyNumberGenotypes[parentIDs.Last()];
if (proband.PhasedGenotype == null)
{
return(IsSharedCnv(canvasSegments, samplesInfo, parentIDs, probandId, maximumCopyNumber));
}
return((proband.PhasedGenotype.ContainsSharedAlleleA(parent1.PhasedGenotype) &&
proband.PhasedGenotype.ContainsSharedAlleleB(parent2.PhasedGenotype)) ||
(proband.PhasedGenotype.ContainsSharedAlleleA(parent2.PhasedGenotype) &&
proband.PhasedGenotype.ContainsSharedAlleleB(parent1.PhasedGenotype)));
}
private static string GetVcfAsString(SampleId sampleId, IEnumerable <ReferencePloidyInterval> intervals, bool symbolicAllele)
{
var vcf =
"##fileformat=VCFv4.1\n" +
$"#CHROM\tPOS\tID\tREF\tALT\tQUAL\tFILTER\tINFO\tFORMAT\tS1\t{sampleId}\tS3\n";
foreach (var interval in intervals)
{
var position = symbolicAllele
? interval.Interval.Interval.OneBasedStart - 1
: interval.Interval.Interval.OneBasedStart;
var altAllele = symbolicAllele ? "<CNV>" : ".";
vcf +=
$"{interval.Interval.Chromosome}\t{position}\t.\tN\t{altAllele}\t.\tPASS\tEND={interval.Interval.Interval.OneBasedEnd}\tCN\t.\t{interval.ReferencePloidy}\t.\n";
}
return(vcf);
}
/// <summary>
/// identify common variants using total CN calls within a pedigree obtained with coverage information only
/// </summary>
/// <param name="canvasSegments"></param>
/// <param name="samplesInfo"></param>
/// <param name="parentIDs"></param>
/// <param name="probandId"></param>
/// <param name="maximumCopyNumber"></param>
/// <returns></returns>
public static bool IsSharedCnv(ISampleMap <CanvasSegment> canvasSegments, ISampleMap <SampleMetrics> samplesInfo, List <SampleId> parentIDs,
SampleId probandId, int maximumCopyNumber)
{
int parent1CopyNumber = Math.Min(canvasSegments[parentIDs.First()].CopyNumber, maximumCopyNumber - 1);
int parent2CopyNumber = Math.Min(canvasSegments[parentIDs.Last()].CopyNumber, maximumCopyNumber - 1);
int probandCopyNumber = Math.Min(canvasSegments[probandId].CopyNumber, maximumCopyNumber - 1);
var parent1Segment = canvasSegments[parentIDs.First()];
var parent2Segment = canvasSegments[parentIDs.Last()];
var probandSegment = canvasSegments[probandId];
int parent1Ploidy = samplesInfo[parentIDs.First()].GetPloidy(parent1Segment);
int parent2Ploidy = samplesInfo[parentIDs.Last()].GetPloidy(parent2Segment);
int probandPloidy = samplesInfo[probandId].GetPloidy(probandSegment);
// Use the following logic: if the proband has fewer copies than expected (from ploidy) but both parents have at least the expected number of copies OR the
// proband has more copies than expected but both parents have no more than the expected number of copies,
// then it is not a 'common CNV' (i.e.it could be de novo); otherwise, it is common
return(!(parent1CopyNumber <= parent1Ploidy && parent2CopyNumber <= parent2Ploidy && probandCopyNumber > probandPloidy ||
parent1CopyNumber >= parent1Ploidy && parent2CopyNumber >= parent2Ploidy && probandCopyNumber < probandPloidy));
}
public static void WriteSegments(string outVcfPath, List <CanvasSegment> segments, double?diploidCoverage,
string wholeGenomeFastaDirectory, string sampleName,
List <string> extraHeaders, PloidyInfo ploidy, int qualityThreshold, bool isPedigreeInfoSupplied, int?denovoQualityThreshold, int?sizeThreshold)
{
using (BgzipOrStreamWriter writer = new BgzipOrStreamWriter(outVcfPath))
{
var genome = WriteVcfHeader(segments, diploidCoverage, wholeGenomeFastaDirectory, new List <string> {
sampleName
},
extraHeaders, writer, qualityThreshold, denovoQualityThreshold, sizeThreshold);
var sampleId = new SampleId(sampleName);
var segmentsOfAllSamples = segments.Select(x => new SampleMap <CanvasSegment> {
{ sampleId, x }
});
WriteVariants(segmentsOfAllSamples, new List <PloidyInfo> {
ploidy
}, genome, writer, denovoQualityThreshold);
}
}
public static SoundEvent Serdes(SoundEvent e, AssetMapping mapping, ISerializer s)
{
if (s == null)
{
throw new ArgumentNullException(nameof(s));
}
e ??= new SoundEvent();
e.Mode = s.EnumU8(nameof(Mode), e.Mode);
e.SoundId = SampleId.SerdesU8(nameof(SoundId), e.SoundId, mapping, s);
e.Unk3 = s.UInt8(nameof(Unk3), e.Unk3);
e.Volume = s.UInt8(nameof(Volume), e.Volume);
e.RestartProbability = s.UInt8(nameof(RestartProbability), e.RestartProbability);
e.FrequencyOverride = s.UInt16(nameof(FrequencyOverride), e.FrequencyOverride);
e.Unk8 = s.UInt16(nameof(Unk8), e.Unk8);
ApiUtil.Assert(e.Unk3 <= 100);
ApiUtil.Assert(e.Volume <= 150);
ApiUtil.Assert(e.RestartProbability <= 102);
ApiUtil.Assert(e.Unk8 == 0);
return(e);
}
public static SoundEvent Serdes(SoundEvent e, AssetMapping mapping, ISerializer s)
{
if (s == null)
{
throw new ArgumentNullException(nameof(s));
}
e ??= new SoundEvent();
e.Mode = s.EnumU8(nameof(Mode), e.Mode);
e.SoundId = SampleId.SerdesU8(nameof(SoundId), e.SoundId, mapping, s);
e.Unk3 = s.UInt8(nameof(Unk3), e.Unk3);
e.Volume = s.UInt8(nameof(Volume), e.Volume);
e.RestartProbability = s.UInt8(nameof(RestartProbability), e.RestartProbability);
e.FrequencyOverride = s.UInt16(nameof(FrequencyOverride), e.FrequencyOverride);
int zeroed = s.UInt16(null, 0);
s.Assert(e.Unk3 <= 100, "SoundEvent: Expected unk3 to be <= 100");
s.Assert(e.Volume <= 150, "SoundEvent: Expected volume to be <= 150");
s.Assert(e.RestartProbability <= 102, "SoundEvent: Expected restart probability to be <= 102");
s.Assert(zeroed == 0, "SoundEvent: Expected field 8 to be 0");
return(e);
}
internal int CallVariants(List <string> variantFrequencyFiles, List <string> segmentFiles,
IFileLocation outVcfFile, string ploidyBedPath, string referenceFolder, List <string> sampleNames, string commonCnvsBedPath, List <SampleType> sampleTypes)
{
// load files
// initialize data structures and classes
var fileCounter = 0;
var samplesInfo = new SampleMap <SampleMetrics>();
var sampleSegments = new SampleMap <Segments>();
var copyNumberModels = new SampleMap <ICopyNumberModel>();
var variantFrequencyFilesSampleList = new SampleMap <string>();
var kinships = new SampleMap <SampleType>();
foreach (string sampleName in sampleNames)
{
var sampleId = new SampleId(sampleName);
var segment = Segments.ReadSegments(_logger, new FileLocation(segmentFiles[fileCounter]));
segment.AddAlleles(CanvasIO.ReadFrequenciesWrapper(_logger, new FileLocation(variantFrequencyFiles[fileCounter]), segment.IntervalsByChromosome));
sampleSegments.Add(sampleId, segment);
var sampleInfo = SampleMetrics.GetSampleInfo(segment.AllSegments, ploidyBedPath, _callerParameters.NumberOfTrimmedBins, sampleId);
var copyNumberModel = _copyNumberModelFactory.CreateModel(_callerParameters.MaximumCopyNumber, sampleInfo.MaxCoverage, sampleInfo.MeanCoverage, sampleInfo.MeanMafCoverage);
samplesInfo.Add(sampleId, sampleInfo);
copyNumberModels.Add(sampleId, copyNumberModel);
variantFrequencyFilesSampleList.Add(sampleId, variantFrequencyFiles[fileCounter]);
kinships.Add(sampleId, sampleTypes[fileCounter]);
fileCounter++;
}
var segmentSetsFromCommonCnvs = CreateSegmentSetsFromCommonCnvs(variantFrequencyFilesSampleList,
_callerParameters.MinAlleleCountsThreshold, commonCnvsBedPath, sampleSegments);
var segmentsForVariantCalling = GetHighestLikelihoodSegments(segmentSetsFromCommonCnvs, samplesInfo, copyNumberModels).ToList();
PedigreeInfo pedigreeInfo = PedigreeInfo.GetPedigreeInfo(kinships, _callerParameters);
Parallel.ForEach(
segmentsForVariantCalling,
new ParallelOptions
{
MaxDegreeOfParallelism = Math.Min(Environment.ProcessorCount, _callerParameters.MaxCoreNumber)
},
segments => _variantCaller.CallVariant(segments, samplesInfo, copyNumberModels, pedigreeInfo)
);
var variantCalledSegments = new SampleMap <List <CanvasSegment> >();
foreach (var key in samplesInfo.SampleIds)
{
variantCalledSegments.Add(key, segmentsForVariantCalling.Select(segment => segment[key]).ToList());
}
var mergedVariantCalledSegments = MergeSegments(variantCalledSegments, _callerParameters.MinimumCallSize, _qualityFilterThreshold);
FilterExcessivelyShortSegments(mergedVariantCalledSegments);
var outputFolder = outVcfFile.Directory;
foreach (var sampleId in samplesInfo.SampleIds)
{
var coverageOutputPath = SingleSampleCallset.GetCoverageAndVariantFrequencyOutput(outputFolder,
sampleId.ToString());
CanvasSegment.WriteCoveragePlotData(mergedVariantCalledSegments[sampleId], samplesInfo[sampleId].MeanCoverage,
samplesInfo[sampleId].Ploidy, coverageOutputPath, referenceFolder);
}
bool isPedigreeInfoSupplied = pedigreeInfo != null && pedigreeInfo.HasFullPedigree();
var denovoQualityThreshold = isPedigreeInfoSupplied ? (int?)_deNovoQualityFilterThreshold : null;
var ploidies = samplesInfo.Select(info => info.Value.Ploidy).ToList();
var diploidCoverage = samplesInfo.Select(info => info.Value.MeanCoverage).ToList();
var names = samplesInfo.SampleIds.Select(id => id.ToString()).ToList();
CanvasSegmentWriter.WriteMultiSampleSegments(outVcfFile.FullName, mergedVariantCalledSegments, diploidCoverage, referenceFolder, names,
null, ploidies, _qualityFilterThreshold, denovoQualityThreshold, CanvasFilter.SegmentSizeCutoff, isPedigreeInfoSupplied);
foreach (var sampleId in samplesInfo.SampleIds)
{
var outputVcfPath = SingleSampleCallset.GetVcfOutput(outputFolder, sampleId.ToString());
var sampleMetrics = samplesInfo[sampleId];
var segments = mergedVariantCalledSegments[sampleId];
CanvasSegmentWriter.WriteSegments(outputVcfPath.FullName, segments,
sampleMetrics.MeanCoverage, referenceFolder, sampleId.ToString(), null,
sampleMetrics.Ploidy, _qualityFilterThreshold, isPedigreeInfoSupplied, denovoQualityThreshold, null);
var visualizationTemp = outputFolder.CreateSubdirectory($"VisualizationTemp{sampleId}");
var normalizationFactor = NormalizationCalculator.ComputeNormalizationFactor(segments);
var bigWig = _coverageBigWigWriter.Write(segments, visualizationTemp, normalizationFactor);
bigWig?.MoveTo(SingleSampleCallset.GetCoverageBigWig(outputFolder, sampleId.ToString()));
var copyNumberBedGraph = SingleSampleCallset.GetCopyNumberBedGraph(outputFolder, sampleId.ToString());
_copyNumberBedGraphWriter.Write(segments, sampleMetrics.Ploidy, copyNumberBedGraph);
var partitionBedgraphHeader = "track type=bedGraph visibility=full autoScale=on graphType=points";
var originalSegments = sampleSegments[sampleId];
_partitionCoverageBedGraphWriter.Write(originalSegments.AllSegments, SingleSampleCallset.GetPartitionBedGraph(outputFolder, sampleId.ToString()), normalizationFactor, partitionBedgraphHeader);
}
return(0);
}
public Sample(SampleId sampleId)
{
var @event = new SampleCreated(sampleId);
Apply(@event);
}
/// <summary>
/// Assess likelihood of a de-novo variant for copyNumberGenotypes configuration with a Mendelian conflict
/// </summary>
/// <param name="canvasSegments"></param>
/// <param name="jointLikelihoods"></param>
/// <param name="parentIDs"></param>
/// <param name="probandId"></param>
/// <param name="samplesInfo"></param>
/// <returns></returns>
internal static double GetConditionalDeNovoQualityScore(ISampleMap <CanvasSegment> canvasSegments, JointLikelihoods jointLikelihoods, ISampleMap <SampleMetrics> samplesInfo, List <SampleId> parentIDs, SampleId probandId)
{
const double q60 = 0.000001;
var parent1Ploidy = Genotype.Create(samplesInfo[parentIDs.First()].GetPloidy(canvasSegments[parentIDs.First()]));
var parent2Ploidy = Genotype.Create(samplesInfo[parentIDs.Last()].GetPloidy(canvasSegments[parentIDs.Last()]));
int probandPloidy = samplesInfo[probandId].GetPloidy(canvasSegments[probandId]);
double deNovoGainMarginalLikelihood = jointLikelihoods.GetMarginalGainDeNovoLikelihood(new KeyValuePair <SampleId, Genotype>(probandId, Genotype.Create(probandPloidy)),
new KeyValuePair <SampleId, Genotype>(parentIDs.First(), parent1Ploidy), new KeyValuePair <SampleId, Genotype>(parentIDs.Last(), parent2Ploidy));
double deNovoLossMarginalLikelihood = jointLikelihoods.GetMarginalLossDeNovoLikelihood(new KeyValuePair <SampleId, Genotype>(probandId, Genotype.Create(probandPloidy)),
new KeyValuePair <SampleId, Genotype>(parentIDs.First(), parent1Ploidy), new KeyValuePair <SampleId, Genotype>(parentIDs.Last(), parent2Ploidy));
double denovoProbability = canvasSegments[probandId].CopyNumber > probandPloidy ?
1 - deNovoGainMarginalLikelihood / (jointLikelihoods.TotalMarginalLikelihood - deNovoLossMarginalLikelihood) :
1 - deNovoLossMarginalLikelihood / (jointLikelihoods.TotalMarginalLikelihood - deNovoGainMarginalLikelihood);
// likelihood of proband genotype != ALT given "copyNumberGenotypes" configuration in pedigree with Mendelian conflict
return(-10.0 * Math.Log10(Math.Max(denovoProbability, q60)));
}
public Sample(SampleId experienceId, Volume volume)
{
Apply(new SampleCreated(experienceId, volume));
}
private static int GetCnState(ISampleMap <CanvasSegment> canvasSegmentsSet, SampleId sampleId, int maximumCopyNumber)
{
return(Math.Min(canvasSegmentsSet[sampleId].CopyNumber, maximumCopyNumber - 1));
}
private bool IsReferenceVariant(ISampleMap <CanvasSegment> canvasSegments, ISampleMap <SampleMetrics> samplesInfo, SampleId sampleId)
{
var segment = canvasSegments[sampleId];
return(GetCnState(canvasSegments, sampleId, _callerParameters.MaximumCopyNumber) == samplesInfo[sampleId].GetPloidy(segment));
}
private bool IsPassVariant(ISampleMap <CanvasSegment> canvasSegments, SampleId sampleId)
{
return(canvasSegments[sampleId].QScore >= _qualityFilterThreshold);
}
public IActionResult DodajanjeAlikvotov([FromForm] DodajanjeAlikvotovViewModel alikvot)
{
if (alikvot == null)
{
return(RedirectToAction("Index"));
}
if (alikvot.IdVzorca == null || alikvot.Box == null)
{
return(View(alikvot));
}
Console.WriteLine(alikvot.IdVzorca);
var originalSample = _dbContext.Samples.Include(m => m.NewId).First(m => m.NewId.ToString() == alikvot.IdVzorca);
var startPosition = 0;
var boxName = _dbContext.Boxes.First(m => m.BoxName == alikvot.Box);
if (alikvot.Location != null)
{
var column = (int)char.GetNumericValue(alikvot.Location[1]);
if (alikvot.Location.Length == 3)
{
column = 10 * column + (int)char.GetNumericValue(alikvot.Location[2]);
}
startPosition = _rowNames[alikvot.Location[0]] * boxName.Size + column;
}
for (var i = 0; i < alikvot.StAlikvotov; i++)
{
var id = new SampleId
{
AliquotSequenceNumber = originalSample.NewId.AliquotSequenceNumber,
IsolateType = originalSample.NewId.IsolateType,
ProcessedAliquotSequenceNumber = originalSample.NewId.ProcessedAliquotSequenceNumber,
ProcessedIsolateType = originalSample.NewId.ProcessedIsolateType,
SequenceNumber = originalSample.NewId.SequenceNumber,
Year = originalSample.NewId.Year
};
if (id.AliquotSequenceNumber == 0)
{
id.AliquotSequenceNumber = i + 1;
}
else
{
id.ProcessedAliquotSequenceNumber = i + 1;
}
if (!alikvot.Volume.HasValue)
{
return(View(alikvot));
}
var sample = new Aliquot
{
Volume = alikvot.VolType == "ml" ? alikvot.Volume.Value : alikvot.Volume.Value / 1000000,
AliquotationDate = alikvot.Date ?? DateTime.Now,
OriginalSample = originalSample,
NewId = id
};
if (alikvot.Project != null)
{
sample.ProjectName = _dbContext.Projects.FirstOrDefault(m => m.Name == alikvot.Project);
}
if (alikvot.Notes != null)
{
sample.Comments = alikvot.Notes;
}
if (!alikvot.Razdelek.HasValue)
{
return(View(alikvot));
}
var stored = new Storage
{
FridgeName = _dbContext.Fridges.FirstOrDefault(m => m.Name == alikvot.Fridge),
Temperature = alikvot.Temp,
Section = alikvot.Razdelek.Value,
BoxName = boxName,
Position = startPosition + i
};
sample.Stored = stored;
_dbContext.Samples.Add(sample);
}
_dbContext.SaveChanges();
return(RedirectToAction("Index"));
}
public SampleCreated(SampleId id)
{
Id = id;
}
public async Task <Sample> Find(SampleId id)
{
return(await Context.Samples.FirstOrDefaultAsync(x => x.DatabaseId == id.Value));
}
public void null_or_whitespace_id_should_be_invalid(string val)
{
var id = new SampleId(val);
Assert.IsFalse(id.IsValid());
}
public OnlyActiveSampleCanBeCancelledRule(SampleId id, bool isDeleted)
{
_id = id;
_isDeleted = isDeleted;
}
public static SampleMetrics GetSampleInfo(IReadOnlyList <CanvasSegment> segments, string ploidyBedPath, int numberOfTrimmedBins, SampleId id)
{
double meanMafCoverage = new SortedList <int>(segments.SelectMany(x => x.Balleles.TotalCoverage)).Median();
double variance = Utilities.Variance(segments.Select(x => x.TruncatedMedianCount(numberOfTrimmedBins)).ToList());
double mafVariance = Utilities.Variance(segments.Where(x => x.Balleles.TotalCoverage.Count > 0)
.Select(x => x.Balleles.TotalCoverage.Average()).ToList());
double meanCoverage = new SortedList <float>(segments.SelectMany(x => x.Counts).Select(x => x)).Median();
int maxCoverage = Convert.ToInt16(segments.Select(x => x.TruncatedMedianCount(numberOfTrimmedBins)).Max()) + 10;
var ploidy = new PloidyInfo();
if (!ploidyBedPath.IsNullOrEmpty() && File.Exists(ploidyBedPath))
{
ploidy = PloidyInfo.LoadPloidyFromVcfFile(ploidyBedPath, id.ToString());
}
return(new SampleMetrics(meanCoverage, meanMafCoverage, variance, mafVariance, maxCoverage, ploidy));
}
public CreateSample(SampleId id)
{
Id = id;
}
public IActionResult DodajanjeVzorca([FromForm] DodajanjeVzorcaViewModel vzorec)
{
if (vzorec == null)
{
return(RedirectToAction("Index"));
}
if (vzorec.Fridge == null || !vzorec.Razdelek.HasValue)
{
return(View(vzorec));
}
Storage stored = new Storage
{
FridgeName = _dbContext.Fridges.FirstOrDefault(m => m.Name == vzorec.Fridge),
Temperature = vzorec.Temp,
Section = vzorec.Razdelek.Value
};
if (vzorec.Location != null && vzorec.Box != null)
{
stored.BoxName = _dbContext.Boxes.First(m => m.BoxName == vzorec.Box);
var column = (int)char.GetNumericValue(vzorec.Location[1]);
if (vzorec.Location.Length == 3)
{
column = 10 * column + (int)char.GetNumericValue(vzorec.Location[2]);
}
stored.Position = _rowNames[vzorec.Location[0]] * stored.BoxName.Size + column;
}
var sampleType = _dbContext.SampleTypes.FirstOrDefault(m => m.SampleTypeName == vzorec.Type);
if (_dbContext.Samples.FirstOrDefault(m =>
m.NewId.Year == (vzorec.DateReception ?? DateTime.Now).Year) == null)
{
_sampleValue = 1;
}
else
{
_sampleValue++;
}
var id = new SampleId
{
AliquotSequenceNumber = 0,
ProcessedAliquotSequenceNumber = 0,
Year = (vzorec.DateReception ?? DateTime.Now).Year,
SequenceNumber = _sampleValue
};
if (sampleType != null)
{
id.IsolateType = sampleType.ShortName;
}
if (!vzorec.Volume.HasValue)
{
return(View(vzorec));
}
var sample = new Sample
{
SubscriberName = vzorec.IdProvider,
Type = sampleType,
Stored = stored,
Accepted = _dbContext.Acceptances.FirstOrDefault(m => m.Date == vzorec.DateReception &&
m.SubscriberName ==
_dbContext.Subscribers.FirstOrDefault(n =>
n.Name == vzorec.Provider)),
NewId = id,
Volume = vzorec.VolType == "ml" ? vzorec.Volume.Value : vzorec.Volume.Value / 1000000
};
if (vzorec.Project != null)
{
sample.ProjectName = _dbContext.Projects.FirstOrDefault(m => m.Name == vzorec.Project);
}
if (vzorec.Notes != null)
{
sample.Comments = vzorec.Notes;
}
if (vzorec.Date != null)
{
sample.Time = vzorec.Date ?? DateTime.Now;
}
_dbContext.Samples.Add(sample);
_dbContext.SaveChanges();
return(RedirectToAction("Index"));
}
