private void CheckPlexType(IsobaricType plexType, string expectHeader, string expectValue)
{
var ann = new IdentifiedSpectrum();
var pqr = new IsobaricItem()
{
PlexType = plexType,
Valid = true,
ValidProbability = 0.0005
};
var refItems = plexType.GetDefinition().Items;
foreach (var item in refItems)
{
pqr[item.Index] = item.Mass;
}
ann.SetIsobaricItem(pqr);
//从实例构建converter
var converter = new ITraqItemPlexConverter<IAnnotation>();
List<IPropertyConverter<IAnnotation>> converters = new List<IPropertyConverter<IAnnotation>>();
converters.Add(converter);
converters.AddRange(converter.GetRelativeConverter(new IAnnotation[] { ann }.ToList()));
CompositePropertyConverter<IAnnotation> finalConverter = new CompositePropertyConverter<IAnnotation>(converters, ',');
if (exportToConsole)
{
Console.WriteLine(finalConverter.Name);
}
Assert.AreEqual(expectHeader, finalConverter.Name);
var line1 = finalConverter.GetProperty(ann);
if (exportToConsole)
{
Console.WriteLine(line1);
}
Assert.AreEqual(expectValue, line1);
var protein2 = new IdentifiedSpectrum();
//从factory根据header构建converter
var finalC = IdentifiedSpectrumPropertyConverterFactory.GetInstance().GetConverters(finalConverter.Name, ',');
finalC.SetProperty(protein2, line1);
var line2 = finalConverter.GetProperty(protein2);
Assert.AreEqual(line1, line2);
}
public void Test()
{
ITraqPhosphoRatioCalculator calc = new ITraqPhosphoRatioCalculator(116, 117, 114, 115);
IsobaricItem item = new IsobaricItem()
{
PlexType = IsobaricType.PLEX4
};
item[114] = 1;
item[115] = 1;
item[116] = 1;
item[117] = 1;
Assert.IsTrue(calc.Valid(item));
item[116] = ITraqConsts.NULL_INTENSITY;
Assert.IsFalse(calc.Valid(item));
item[116] = 1;
item[117] = ITraqConsts.NULL_INTENSITY;
Assert.IsFalse(calc.Valid(item));
}
public void Test()
{
ITraqPhosphoRatioCalculator calc = new ITraqPhosphoRatioCalculator(116, 117, 114, 115);
IsobaricItem item = new IsobaricItem()
{
PlexType = IsobaricType.PLEX4
};
item[114] = 1;
item[115] = 1;
item[116] = 1;
item[117] = 1;
Assert.IsTrue(calc.Valid(item));
item[116] = ITraqConsts.NULL_INTENSITY;
Assert.IsFalse(calc.Valid(item));
item[116] = 1;
item[117] = ITraqConsts.NULL_INTENSITY;
Assert.IsFalse(calc.Valid(item));
}
public void Setup()
{
t1 = new IsobaricItem()
{
Experimental = "S1",
ScanMode = "HCD",
PlexType = IsobaricType.PLEX4,
PrecursorPercentage = 0.85,
Scan = new ScanTime(255, 3.4),
RawPeaks = new PeakList<Peak>(new Peak[] { new Peak(114.1, 114.1), new Peak(115.1, 115.1), new Peak(116.1, 116.1), new Peak(117.1, 117.1) }.ToList()),
PeakInIsolationWindow = new PeakList<Peak>(new Peak[] { new Peak(214.1, 114.1), new Peak(215.1, 115.1), new Peak(216.1, 116.1), new Peak(217.1, 117.1) }.ToList())
{
Precursor = new PrecursorPeak()
{
MasterScan = 1,
Charge = 2,
Intensity = 3.0,
IsolationMass = 1800.1,
IsolationWidth = 2.0,
MonoIsotopicMass = 1879.1
}
}
};
t1[114] = 4.5;
t1[115] = 5.5;
t1[116] = 6.5;
t1[117] = 7.5;
t2 = new IsobaricItem()
{
Experimental = "S2",
ScanMode = "ETD",
PlexType = IsobaricType.PLEX8,
PrecursorPercentage = 0.33,
Scan = new ScanTime(355, 4.4),
RawPeaks = new PeakList<Peak>(new Peak[] { new Peak(114.1, 1114.1), new Peak(115.1, 1115.1), new Peak(116.1, 1116.1), new Peak(117.1, 1117.1) }.ToList()),
PeakInIsolationWindow = new PeakList<Peak>(new Peak[] { new Peak(1214.1, 114.1), new Peak(1215.1, 115.1), new Peak(1216.1, 116.1), new Peak(1217.1, 117.1) }.ToList())
{
Precursor = new PrecursorPeak()
{
MasterScan = 3,
Charge = 3,
Intensity = 4.0,
IsolationMass = 2800.1,
IsolationWidth = 3.0,
MonoIsotopicMass = 2879.1
}
}
};
t2[113] = 3.5;
t2[114] = 4.5;
t2[115] = 5.5;
t2[116] = 6.5;
t2[117] = 7.5;
t2[118] = 8.5;
t2[119] = 9.5;
t2[121] = 11.5;
tr = new IsobaricResult();
tr.Mode = "PQD";
tr.Add(t1);
tr.Add(t2);
}
