public Form1()
{
InitializeComponent();
rbProfTfsMhdac.Visible = false;
#if ENABLE_MHDAC_TESTS
rbProfTfsMhdac.Visible = true;
#endif
rbProfTfsIter.Visible = false;
rbFrameMsIter.Visible = false;
rbFrameMsArrayOfIters.Visible = false;
lbFileContents.Text = "No file is yet opened.";
lbFileContents2.Text = "";
gbCCS.Enabled = false;
lbStatus.Text = "";
// m_currentFilePath = @"D:\Data\Asms_Demo\KnitFile.d";
m_currentFilePath = @"C:\Assembly\Test\TestData\ImsSynthChrom.d";
m_reader = null;
rbAllIonsHigh.Enabled = false;
rbAllIonsLow.Enabled = false;
rbAllIonsAll.Enabled = false;
lbStatusAllIons.Visible = false;
#if PNNL_VERSION
// placeholder for internal tests
rbFrameMsArrayOfIters.Visible = true;
#endif
}
private void cbBrowse_Click(object sender, EventArgs e)
{
if (m_reader != null)
{
m_reader.Close();
}
m_reader = null;
BrowseForInputFile();
}
private void cbOpen_Click(object sender, EventArgs e)
{
if (string.IsNullOrEmpty(txtFilePath.Text))
{
MessageBox.Show("No input file is specified");
return;
}
string filePath = txtFilePath.Text.Trim();
if (!Directory.Exists(filePath))
{
MessageBox.Show(string.Format("Folder {0} does not exist.", filePath));
return;
}
if (!MidacFileAccess.FileHasImsData(filePath))
{
lbFileContents.Text = "File does not have IM-MS data (not opened)";
lbFileContents2.Text = "";
gbCCS.Enabled = false;
return;
}
if (m_reader != null)
{
m_reader.Close();
}
m_reader = null;
// Returns a reader alredy opened to the specified file path
m_reader = MidacFileAccess.ImsDataReader(filePath);
m_currentFilePath = filePath;
// Get overall file metadata
IMidacFileInfo fileInfo = m_reader.FileInfo;
// show something about the contents of the file
string tfsType = "";
if (m_reader.HasPeakTfsSpectra)
{
tfsType += "Peak";
}
if (m_reader.HasProfileTfsSpectra)
{
tfsType += tfsType.Length > 0 ? " and Profile" : "Profile";
}
lbFileContents.Text = string.Format("{0} frames with max of {1} drift bins/frame; with {2} Total Frame Spectra for each frame",
fileInfo.NumFrames,
fileInfo.MaxNonTfsMsPerFrame,
tfsType);
gbCCS.Enabled = m_reader.HasSingleFieldCcsInformation;
lbFileContents2.Text = string.Format("{0} prepared for CCS conversions", m_reader.HasSingleFieldCcsInformation ? "Is" : "Is NOT");
// If there are high/low fragmentation frames, find out how many
if (fileInfo.HasHiLoFragData)
{
IMidacMsFiltersChrom chromFilters = MidacFileAccess.DefaultMsChromFilters;
chromFilters.ApplicableFilters = ApplicableFilters.FragmentationClass;
chromFilters.FragmentationClass = FragmentationClass.HighEnergy;
int numHighFrames = m_reader.FilteredFrameNumbers(chromFilters).Length;
chromFilters.FragmentationClass = FragmentationClass.LowEnergy;
int numLowFrames = m_reader.FilteredFrameNumbers(chromFilters).Length;
rbAllIonsAll.Enabled = true;
rbAllIonsHigh.Enabled = true;
rbAllIonsLow.Enabled = true;
lbStatusAllIons.Visible = true;
lbStatusAllIons.Text = string.Format("{0} High-CE frames; {1} Low-CE frames", numHighFrames, numLowFrames);
}
else
{
rbAllIonsAll.Enabled = false;
rbAllIonsHigh.Enabled = false;
rbAllIonsLow.Enabled = false;
lbStatusAllIons.Visible = false;
}
// Force read of some data before we start; gets some assertions out of the way.
IMidacUnitConverter converter = m_reader.FrameInfo(1).FrameUnitConverter;
}
