/// <summary>
/// Convert image to color space of known stain.
/// </summary>
/// <param name="source">Source image.</param>
/// <param name="stain">Known stain.</param>
/// <param name="channel">Channel of stain. Takes int 0-2.</param>
/// <returns>Color deconvoluted image.</returns>
public static Bitmap ColorDeconvolution(Bitmap source, ColorDeconvolution.KnownStain stain, int channel)
{
using (var image = source.Clone() as Bitmap)
{
BitmapProcessor bitmapProcessor = new BitmapProcessor(source);
ColorDeconvolution colorDeconvolution = new ColorDeconvolution();
GrayscaleProcessor gpDeconvoluted = null;
if (channel == 0)
{
gpDeconvoluted = colorDeconvolution.Get1stStain(bitmapProcessor, stain);
}
else if (channel == 1)
{
gpDeconvoluted = colorDeconvolution.Get2ndStain(bitmapProcessor, stain);
}
else if (channel == 2)
{
gpDeconvoluted = colorDeconvolution.Get3rdStain(bitmapProcessor, stain);
}
else
{
return(null);
}
Bitmap result = gpDeconvoluted.Bitmap.Clone() as Bitmap;
gpDeconvoluted.Dispose();
return(result);
}
}
/// <summary>
/// Get color channel of a known stain.
/// </summary>
/// <param name="image">input image</param>
/// <param name="stain">known stain</param>
/// <param name="channel">channel of the known stain</param>
/// <returns>color channel</returns>
public static UMat GetColorDeconvolutedChannelAsUMat(Bitmap image, ColorDeconvolution.KnownStain stain, int channel)
{
Bitmap copy = (Bitmap)image.Clone();
Bitmap bitmap = null;
UMat umat = null;
try
{
bitmap = ColorDeconvolution(copy, stain, channel);
umat = new Image <Gray, byte>(bitmap).ToUMat();
} catch (Exception ex)
{
System.Windows.Forms.MessageBox.Show(ex.Message);
} finally
{
if (bitmap != null)
{
bitmap.Dispose();
}
}
return(umat);
}
