public void KeepOriginalIfAlignmentUnchanged()
{
// If realignment results in same alignment, don't count it as realigned
// If realignment results in different cigar and same position, count it as realigned
// If realignment results in same cigar and different position, count it as realigned -- having trouble recreating this situation...
var hasIndels = new Read("chr", CreateAlignment("hasIndels", 1, "2M2I3M", "ZATTAAZ"));
var hasIndels2 = new Read("chr", CreateAlignment("hasIndels2", 1, "2M2I3M", "ZATTAAZ"));
var realignSamePosNewCigar = new Read("chr", CreateAlignment("candidateForRealignmentGoesToSamePos", 1, "7M", "ZATTAAZ"));
var extractorForRealign = new MockExtractor(new List <Read>
{
hasIndels,
hasIndels2,
realignSamePosNewCigar,
});
var extractorForCandidates = new MockExtractor(new List <Read>
{
hasIndels,
hasIndels2,
realignSamePosNewCigar,
});
var writer = new MockRealignmentWriter(new List <string>
{
realignSamePosNewCigar.Name,
// the reads that are expected to be re-aligned
}, new List <string>
{
hasIndels.Name,
hasIndels2.Name
// reads that are expected to be written but not re-aligned
});
SetupExecute(extractorForRealign, extractorForCandidates, writer, true, 50, "YZAAAZZZZZZZ");
}
public void CandidateExtractFilter()
{
// do not take candidates from reads with MapQ of zero, or secondary alignments
var highMapQ = new Read("chr", CreateAlignment("highMapQ", 0, "2M2I3M", "AAGGAAA"));
var lowMapQ = new Read("chr", CreateAlignment("lowMapQ", 0, "2M2I3M", "AATTAAA", mapq: 0));
var secondaryRead = new Read("chr", CreateAlignment("secondaryRead", 0, "2M2I3M", "AACCAAA"));
secondaryRead.BamAlignment.SetIsSecondaryAlignment(true);
var testIndel1 = new Read("chr", CreateAlignment("testIndel1", 0, "7M", "AAGGAAA"));
var testIndel2 = new Read("chr", CreateAlignment("testIndel2", 0, "7M", "AATTAAA"));
var testIndel3 = new Read("chr", CreateAlignment("testIndel2", 0, "7M", "AACCAAA"));
var extractorForRealign = new MockExtractor(new List <Read>
{
highMapQ,
lowMapQ,
secondaryRead,
testIndel1,
testIndel2,
testIndel3
});
var extractorForCandidates = new MockExtractor(new List <Read>
{
highMapQ,
lowMapQ,
secondaryRead,
testIndel1,
testIndel2,
testIndel3
});
var writer = new MockRealignmentWriter(new List <string>
{
// reads that are expected to be re-aligned
testIndel1.Name
}, new List <string>
{
// reads that are expected to be written but not re-aligned
highMapQ.Name,
lowMapQ.Name,
secondaryRead.Name,
testIndel2.Name,
testIndel3.Name
}
);
SetupExecute(extractorForRealign, extractorForCandidates, writer, true, 50,
allowRescoringOrig0: true, chrReference: "AAAAAAAAAAAAAAAAAAAAAAAAAA");
}
public void SkipRemoveDuplicatesTrue_VariantBelowThreshold()
{
// 1 unique read and 1 duplicate read have indels
// With duplicates not considered in the evidence counting
// the frequency in this case should be 1 (uniq read with indel) / 3 (total uniq reads) = 33.3333333%
var hasIndels = new Read("chr", CreateAlignment("hasIndels", 0, "2M1I3M", "AATAAA"));
var uniqRef = new Read("chr", CreateAlignment("uniqRef", 0, "6M", "AAAAAA"));
var uniqCandidate = new Read("chr", CreateAlignment("uniqCandidate", 0, "6M", "AATAAA"));
var dupWithIndel = new Read("chr", CreateAlignment("dupWithIndels", 0, "2M1I3M", "AATAAA"));
var dupRef = new Read("chr", CreateAlignment("dubRef", 0, "6M", "AAAAAA"));
dupWithIndel.BamAlignment.SetIsDuplicate(true);
dupRef.BamAlignment.SetIsDuplicate(true);
// don't allow dups
var extractorForRealign2 = new MockExtractor(new List <Read>
{
uniqRef,
hasIndels,
uniqCandidate,
dupWithIndel,
dupRef
});
var extractorForCandidates2 = new MockExtractor(new List <Read>
{
uniqRef,
hasIndels,
uniqCandidate,
dupWithIndel,
dupRef
});
var writerBelowThreshold = new MockRealignmentWriter(new List <string>
{
// the reads that are expected to be re-aligned
}, new List <string>
{
// reads that are expected to be written but not re-aligned
uniqCandidate.Name, hasIndels.Name, uniqRef.Name,
});
// when the threshold is set to 0.34 (34%) this case falls below with 33.33333%
// No realignment should be triggered
SetupExecute(extractorForRealign2, extractorForCandidates2, writerBelowThreshold, false, 50, "AAAAAAAAAAA", 0.34f, true);
}
public void SkipDuplicatesFalse_VariantAboveThreshold()
{
// 1 unique read and 1 duplicate read have indels
// With duplicates considered in the evidence counting
// the frequency in this case should be 2 (uniq & dup reads with indel) / 5 (total reads) = 40%
var hasIndels = new Read("chr", CreateAlignment("hasIndels", 0, "2M2I3M", "AATTAAA"));
var uniqRef = new Read("chr", CreateAlignment("uniqRef", 0, "7M", "AAAAAAA"));
var uniqCandidate = new Read("chr", CreateAlignment("uniqCandidate", 0, "7M", "AATTAAA"));
var dupWithIndel = new Read("chr", CreateAlignment("dupWithIndels", 0, "2M2I3M", "AATTAAA"));
var dupRef = new Read("chr", CreateAlignment("dubRef", 0, "7M", "AAAAAAA"));
dupWithIndel.BamAlignment.SetIsDuplicate(true);
dupRef.BamAlignment.SetIsDuplicate(true);
var extractorForRealign = new MockExtractor(new List <Read>
{
uniqRef,
hasIndels,
uniqCandidate,
dupWithIndel,
dupRef
});
var extractorForCandidates = new MockExtractor(new List <Read>
{
uniqRef,
hasIndels,
uniqCandidate,
dupWithIndel,
dupRef
});
var writer = new MockRealignmentWriter(new List <string>
{
uniqCandidate.Name
}, new List <string>
{
hasIndels.Name, dupWithIndel.Name,
uniqRef.Name, dupRef.Name,
});
// The variant frequency (40%) in this case is above the threshold 0.39 (39%)
// Realignment of candidate reads should be triggered
SetupExecute(extractorForRealign, extractorForCandidates, writer, false, 50, "AAAAAAAAAAA", 0.39f);
}
private void RealignAndCheckQuality(uint initialQuality, bool hasMismatches, uint expectedQuality, bool allowRescoringOrig0 = true)
{
var hasIndels = new Read("chr", CreateAlignment("hasIndels", 0, "2M4I3M", "AATTTTAAA"));
var candidateForRealignment = new Read("chr", CreateAlignment("uniqCandidate", 0, "9M", hasMismatches ? "AATTTTAAZ" : "AATTTTAAA"));
candidateForRealignment.BamAlignment.MapQuality = initialQuality;
var extractorForRealign = new MockExtractor(new List <Read>
{
hasIndels,
candidateForRealignment,
});
var extractorForCandidates = new MockExtractor(new List <Read>
{
hasIndels,
candidateForRealignment,
});
var writer = new MockRealignmentWriter(new List <string>
{
candidateForRealignment.Name,
// the reads that are expected to be re-aligned
}, new List <string>
{
hasIndels.Name
// reads that are expected to be written but not re-aligned
});
SetupExecute(extractorForRealign, extractorForCandidates, writer, true, 50,
allowRescoringOrig0: allowRescoringOrig0,
chrReference: "AAAAAAAAAAAAAAAAAAAAAAAAAA", verifyRemappedReads: (reads) =>
{
foreach (var read in reads)
{
if (read.Name == candidateForRealignment.Name)
{
Assert.Equal(expectedQuality, read.MapQuality);
}
}
});
}
public void UpdateNMTag()
{
// should not be realigned and wrong NM will be kept
var hasIndelsWrongNM = new Read("chr", CreateAlignment("hasIndelsWrongNM", 0, "2M4I3M", "AATTTTAAA", 2));
var expectZeroMismatch = new Read("chr", CreateAlignment("expectZeroMismatch", 0, "9M", "AATTTTAAA", 1));
var expectOneMismatch = new Read("chr", CreateAlignment("expectOneMismatch", 0, "9M", "AATTTTAAC", 2));
// realignment will fix wrong NM
var ExpectOneMismatchWrongNM = new Read("chr", CreateAlignment("ExpectOneMismatchWrongNM", 0, "9M", "AATTTTAAC", 0));
// No NM tag to start with and new NM tag will be added
var noNMTag = new Read("chr", CreateAlignment("noNMTag", 0, "9M", "AATTTTAAC"));
var extractorForRealign = new MockExtractor(new List <Read>
{
hasIndelsWrongNM,
expectZeroMismatch,
expectOneMismatch,
ExpectOneMismatchWrongNM,
noNMTag
});
var extractorForCandidates = new MockExtractor(new List <Read>
{
hasIndelsWrongNM,
expectZeroMismatch,
expectOneMismatch,
ExpectOneMismatchWrongNM,
noNMTag
});
var writer = new MockRealignmentWriter(new List <string>
{
// reads that are expected to be re-aligned
expectZeroMismatch.Name,
expectOneMismatch.Name,
ExpectOneMismatchWrongNM.Name,
noNMTag.Name
}, new List <string>
{
// reads that are expected to be written but not re-aligned
hasIndelsWrongNM.Name
}
);
SetupExecute(extractorForRealign, extractorForCandidates, writer, true, 50,
allowRescoringOrig0: true,
chrReference: "AAAAAAAAAAAAAAAAAAAAAAAAAA", verifyRemappedReads: (reads) =>
{
foreach (var read in reads)
{
if (read.Name == hasIndelsWrongNM.Name)
{
Assert.Equal(2, read.GetIntTag("NM"));
}
if (read.Name == expectZeroMismatch.Name)
{
Assert.Equal(4, read.GetIntTag("NM"));
}
if (read.Name == expectOneMismatch.Name)
{
Assert.Equal(5, read.GetIntTag("NM"));
}
if (read.Name == ExpectOneMismatchWrongNM.Name)
{
Assert.Equal(5, read.GetIntTag("NM"));
}
if (read.Name == noNMTag.Name)
{
Assert.Equal(null, read.GetIntTag("NM"));
}
}
});
}
public void Execute()
{
var dupRead = new Read("chr", CreateAlignment("Duplicate", 5, "5S5M5I5M", "ACGTACGTACTATATAATAC"));
dupRead.BamAlignment.SetIsDuplicate(true);
var nonPrimaryRead = new Read("chr", CreateAlignment("NonPrimary", 5, "5S5M5I5M", "ACGTACGTACTATATAATAC"));
nonPrimaryRead.BamAlignment.SetIsSecondaryAlignment(true);
var SupplementaryRead = new Read("chr", CreateAlignment("Supplementary", 5, "5S5M5I5M", "ACGTACGTACTATATAATAC"));
SupplementaryRead.BamAlignment.SetIsSupplementaryAlignment(true);
var HasSupplementaryRead = new Read("chr", CreateAlignment("HasSupplementary", 5, "5S5M5I5M", "ACGTACGTACTATATAATAC"));
var tagUtils = new TagUtils();
tagUtils.AddStringTag("SA", "dummy");
HasSupplementaryRead.BamAlignment.AppendTagData(tagUtils.ToBytes());
var passesSuspicion = new Read("chr", CreateAlignment("PassesSuspicion", 0, "4M", "ACGT"));
var hasIndels = new Read("chr", CreateAlignment("HasIndels", 5, "5S5M5I5M", "ACGTACGTACTATATAATAC"));
// HasIndels shifts too far. Do not write it. Don't realign dups.
var extractorForRealign = new MockExtractor(new List <Read>
{
CopyRead(dupRead),
CopyRead(nonPrimaryRead),
CopyRead(SupplementaryRead),
CopyRead(HasSupplementaryRead),
CopyRead(passesSuspicion),
CopyRead(hasIndels)
});
var writer = new MockRealignmentWriter(new List <string>
{
}, new List <string>
{
hasIndels.Name, nonPrimaryRead.Name, SupplementaryRead.Name, HasSupplementaryRead.Name, dupRead.Name, passesSuspicion.Name
});
SetupMocksandExecute(extractorForRealign, writer, true, 2);
// Allow realignment of dups, and increase max shift to let hasIndels through
extractorForRealign = new MockExtractor(new List <Read>
{
CopyRead(dupRead),
CopyRead(nonPrimaryRead),
CopyRead(SupplementaryRead),
CopyRead(HasSupplementaryRead),
CopyRead(passesSuspicion),
CopyRead(hasIndels)
});
writer = new MockRealignmentWriter(new List <string>
{
dupRead.Name,
hasIndels.Name
}, new List <string>
{
nonPrimaryRead.Name, SupplementaryRead.Name, HasSupplementaryRead.Name, passesSuspicion.Name
});
SetupMocksandExecute(extractorForRealign, writer, false, 50);
//Don't allow dups, but make max shift big enough for hasIndels
extractorForRealign = new MockExtractor(new List <Read>
{
CopyRead(dupRead),
CopyRead(nonPrimaryRead),
CopyRead(SupplementaryRead),
CopyRead(HasSupplementaryRead),
CopyRead(passesSuspicion),
CopyRead(hasIndels)
});
writer = new MockRealignmentWriter(new List <string>
{
hasIndels.Name,
}, new List <string>
{
dupRead.Name, nonPrimaryRead.Name, SupplementaryRead.Name, HasSupplementaryRead.Name, passesSuspicion.Name
});
SetupMocksandExecute(extractorForRealign, writer, true, 50);
}