public List<string> GeneratePooling(int sampleCount,ref List<string> barcodesTrace)
{
//first process batchs,48 per batch for 6 pooling into 1, 64 for 8 pooling into 1,etc.
int sampleCntPerBatch = 8 * GlobalVars.Instance.PoolingCnt;
int batchCnt = sampleCount / sampleCntPerBatch;
List<PipettingInfo> batchPipettingInfos = new List<PipettingInfo>();
int startGridID = GlobalVars.Instance.StartGridID;//
List<string> strs = new List<string>();
for (int i = 0; i < batchCnt; i++)
{
var thisGridPipettingInfos = GenerateBatch(startGridID);
strs.AddRange(Format(thisGridPipettingInfos));
strs.Add(string.Format("B;Comment(\"Pippeting Batch: {0}\");", i + 1));
batchPipettingInfos.AddRange(thisGridPipettingInfos);
curDstWellStartIndex += 8;
startGridID += 3;
}
strs.Add("B;Comment(\"Pippeting Remaining\");");
//process remaining without addtional neg
#region noneedAdditional
List<PipettingInfo> fragmentsPipettingInfo = new List<PipettingInfo>();
int remainingCnt = sampleCount - batchCnt * sampleCntPerBatch;
int dstWellCntNeeded = remainingCnt / GlobalVars.Instance.PoolingCnt;
int noNeedAdditionalCnt = dstWellCntNeeded * GlobalVars.Instance.PoolingCnt;
for (int wellIndex = 0; wellIndex < noNeedAdditionalCnt; wellIndex++)
{
int srcGridID = startGridID + wellIndex / 16;
int wellIndexInGrid = wellIndex - (wellIndex / 16) * 16;
int dstWellIndex = wellIndex - wellIndex / dstWellCntNeeded * dstWellCntNeeded;
string sGrid = string.Format("grid{0}", srcGridID);
Position pos = new Position(srcGridID - 1, wellIndexInGrid);
if (!GlobalVars.Instance.pos_BarcodeDict.ContainsKey(pos))
throw new Exception(string.Format("Cannot find barcode for grid:{0}, pos:{1}", pos.x, pos.y));
string barcode = GlobalVars.Instance.pos_BarcodeDict[pos];
int dstWellID = curDstWellStartIndex + dstWellIndex + 1;
double volume = Math.Round(GlobalVars.Instance.PipettingVolume / 2, 1);
fragmentsPipettingInfo.Add(new PipettingInfo(
sGrid,
wellIndexInGrid + 1,
volume,
GlobalVars.Instance.DstLabware,
MapDstWellID(dstWellID),
barcode));
fragmentsPipettingInfo.Add(new PipettingInfo(
sGrid,
wellIndexInGrid + 1,
volume,
GetSecondSliceDstLabware(),
MapDstWellID(dstWellID, false),
barcode));
}
curDstWellStartIndex += dstWellCntNeeded;
#endregion
//process remaining
remainingCnt -= dstWellCntNeeded * GlobalVars.Instance.PoolingCnt;
dstWellCntNeeded = CalculateNeededDstWell(remainingCnt);
int additionalWellCnt = dstWellCntNeeded * GlobalVars.Instance.PoolingCnt - remainingCnt;
for (int wellIndex = 0; wellIndex < remainingCnt + additionalWellCnt; wellIndex++ )
{
int wellIndexInBatch = wellIndex + noNeedAdditionalCnt;
int srcGridID = startGridID + wellIndexInBatch / 16;
int wellIndexInGrid = wellIndexInBatch - (wellIndexInBatch / 16) * 16;
int dstWellIndex = wellIndex - wellIndex / dstWellCntNeeded * dstWellCntNeeded;
string sGrid = string.Format("grid{0}", srcGridID);
string barcode = "";
if (wellIndex >= remainingCnt)
continue;
var pos = new Position(srcGridID - 1, wellIndexInGrid);
if (!GlobalVars.Instance.pos_BarcodeDict.ContainsKey(pos))
throw new Exception(string.Format("Cannot find barcode for grid:{0}, pos:{1}", pos.x, pos.y));
barcode = GlobalVars.Instance.pos_BarcodeDict[pos];
int dstWellID = curDstWellStartIndex + dstWellIndex + 1;
double volume = Math.Round(GlobalVars.Instance.PipettingVolume/2,1);
fragmentsPipettingInfo.Add(new PipettingInfo(
sGrid,
wellIndexInGrid + 1,
volume,
GlobalVars.Instance.DstLabware,
MapDstWellID(dstWellID),
barcode ));
fragmentsPipettingInfo.Add(new PipettingInfo(
sGrid,
wellIndexInGrid + 1,
volume,
GetSecondSliceDstLabware(),
MapDstWellID(dstWellID,false),
barcode));
}
curDstWellStartIndex += dstWellCntNeeded;
strs.AddRange(Format(fragmentsPipettingInfo));
batchPipettingInfos.AddRange(fragmentsPipettingInfo);
barcodesTrace = GetBarcodesSourceInfo(batchPipettingInfos);
return strs;
}
private List<string> GenerateNormal(int poolingSampleCnt, int normalSampleCnt, ref List<string> normalBarcodeTrace)
{
int startGridID = GlobalVars.Instance.StartGridID;//
List<PipettingInfo> normalPipettings = new List<PipettingInfo>();
for (int i = 0; i < normalSampleCnt; i++)
{
int wellIndex = i + poolingSampleCnt;
int srcGridID = startGridID + wellIndex / 16;
int wellIndexInGrid = wellIndex - (wellIndex / 16) * 16;
string sGrid = string.Format("grid{0}", srcGridID);
int dstWellID = curDstWellStartIndex + i + 1;
Position pos = new Position(srcGridID - 1, wellIndexInGrid);
if (!GlobalVars.Instance.pos_BarcodeDict.ContainsKey(pos))
throw new Exception(string.Format("Cannot find barcode for grid:{0}, pos:{1}", pos.x, pos.y));
string barcode = GlobalVars.Instance.pos_BarcodeDict[pos];
double volume = 480;//Math.Round(GlobalVars.Instance.PipettingVolume / 2, 1);
normalPipettings.Add(new PipettingInfo(
sGrid,
wellIndexInGrid + 1,
volume,
GlobalVars.Instance.DstLabware,
MapDstWellID(dstWellID),
barcode));
normalPipettings.Add(new PipettingInfo(
sGrid,
wellIndexInGrid + 1,
volume,
GetSecondSliceDstLabware(),
MapDstWellID(dstWellID,false),
barcode));
}
curDstWellStartIndex += normalSampleCnt;
List<string> strs = new List<string>();
strs.AddRange(Format(normalPipettings));
normalBarcodeTrace = GetBarcodesSourceInfo(normalPipettings);
return strs;
}
internal void SetBarcodes(int gridID, List<string> barcodes)
{
int gridIndex = gridID - 1;
for(int i = 0; i< barcodes.Count; i++)
{
//if (barcodes[i] == "$$$")
// continue;
Position pos = new Position(gridIndex, i);
if (pos_BarcodeDict.ContainsKey(pos))
pos_BarcodeDict[pos] = barcodes[i];
else
pos_BarcodeDict.Add(pos, barcodes[i]);
}
}