private void _RenderMicroImage()
{
MicroImage microImage = radioButtonPic473.Checked ? this._GetCurrentMicroImage("473") : this._GetCurrentMicroImage("561");
Image image;
if (checkBinarize.Checked == true)
{
int threshold = (int)nudThreshold.Value;
image = microImage.GetBinarizedImage(threshold);
}
else
{
image = microImage.GetImage();
}
if (checkEnabled.Checked == true && nudX.Value > 0 && nudY.Value > 0 && nudR.Value > 0)
{
int x = (int)nudX.Value;
int y = (int)nudY.Value;
int r = (int)nudR.Value;
Image tmp = new Bitmap(image.Width, image.Height);
Graphics g = Graphics.FromImage(tmp);
g.DrawImage(image, 0, 0, image.Width, image.Height);
Pen pen = new Pen(Color.FromArgb(191, Color.LightGray), this._Project.X / 200);
g.DrawEllipse(pen, x - r, y - r, r * 2, r * 2);
g.Dispose();
image = tmp;
}
pictureMicroImage.Image = image;
}
public Analyzer(Project project, int c)
{
this._Project = project;
this.C = c;
MicroImage[,] microImages = new MicroImage[this._Project.Z, this._Project.T];
MicroImage[,] microImages561 = new MicroImage[this._Project.Z, this._Project.T];
for (int z = 1; z <= this._Project.Z; z++)
{
for (int t = 1; t <= this._Project.T; t++)
{
string file = this._Project.GetImageFile(this.C, z, t, "473");
string file561 = this._Project.GetImageFile(this.C, z, t, "561");
microImages[z - 1, t - 1] = new MicroImage(file, this.C, z, t);
microImages561[z - 1, t - 1] = new MicroImage(file561, this.C, z, t);
}
}
this.MicroImages = microImages;
this.MicroImages561 = microImages561;
}
public double[] AnalyzeSpot(AnalyzeAreaSet analyzeAreaSet, int t, int threshold)
{
int targetZ = 0;
//AnalyzeArea analyzeArea = analyzeAreaSet.GetAnalyzeArea(1, t);
AnalyzeArea analyzeArea = new AnalyzeArea();
for (int z = 1; z <= this._Project.Z; z++)
{
analyzeArea = analyzeAreaSet.GetAnalyzeArea(z, t);
if (analyzeArea.Enabled)
{
targetZ = z;
break;
}
}
if (targetZ == 0)
{
return(new double[3]);
}
double[] spots = new double[3];
MicroImage microImage473 = this.GetMicroImage(targetZ, t, "473");
MicroImage microImage561 = this.GetMicroImage(targetZ, t, "561");
//spots[0] = analyzeArea.Count473;
//spots[1] = analyzeArea.Count561;
spots[0] = microImage473.AnalyzeSpot(analyzeArea, threshold);
spots[1] = microImage561.AnalyzeSpot(analyzeArea, threshold);
//Integrated Density
spots[2] = microImage473.AnalyzeLuminance(analyzeArea, threshold);
return(spots);
}
private FillSource _Fill(int z, int t, FillSource source, int threshold)
{
MicroImage microImage = this._Analyzer.GetMicroImage(z, t, "473");
//MicroImage microImage561 = this._Analyzer.GetMicroImage(z, t, "561");
Bitmap image = microImage.GetBinarizedImage(threshold);
//Bitmap image561 = microImage561.GetBinarizedImage(threshold);
ConnectedComponents components = ConnectedComponents.Analyze(image);
//ConnectedComponents components561 = ConnectedComponents.Analyze(image561);
Dictionary <int, int> labels = new Dictionary <int, int>();
int sMinX = (source.MinX < 1) ? 0 : source.MinX;
int sMaxX = (source.MaxX < 1) ? 0 : source.MaxX;
int sMinY = (source.MinY < 1) ? 0 : source.MinY;
int sMaxY = (source.MaxY < 1) ? 0 : source.MaxY;
if (source.Label > 0)
{
for (int x = sMinX; x <= sMaxX; x++)
{
for (int y = sMinY; y <= sMaxY; y++)
{
int label = components.Labels[x, y];
if (label <= 0)
{
continue;
}
if (source.Components.Labels[x, y] == source.Label)
{
if (labels.ContainsKey(label))
{
labels[label]++;
}
else
{
labels[label] = 1;
}
}
}
}
}
int max = 0;
foreach (KeyValuePair <int, int> label in labels)
{
if (max == 0)
{
max = label.Key;
}
else if (label.Value >= labels[max]) // TODO: 等しい場合の処理 中心が近いものなど
{
max = label.Key;
}
}
nudT.Value = t;
FillSource newSource = new FillSource(components, max);
if (max == 0)
{
checkEnabled.Checked = false;
}
else
{
int xLength = newSource.MaxX - newSource.MinX;
int yLength = newSource.MaxY - newSource.MinY;
int longer = xLength > yLength ? xLength : yLength;
checkEnabled.Checked = true;
nudX.Value = newSource.MinX + (int)(xLength / 2);
nudY.Value = newSource.MinY + (int)(yLength / 2);
if (nudR.Maximum < ((int)(longer / 2) + 3))
{
nudR.Value = nudR.Maximum;
}
else
{
nudR.Value = (int)(longer / 2) + 3; // TODO: 3 shold be defined some where else.
}
nudThreshold.Value = threshold;
}
////*************************** SPOT 解析 ***************************
////選択部分の取得
//Bitmap imageEllipse = new Bitmap(image.Width, image.Height);
//Graphics g = Graphics.FromImage(imageEllipse);
//g.Clear(Color.Black);
//g.FillEllipse(Brushes.White, (int)nudX.Value - (int)nudR.Value, (int)nudY.Value - (int)nudR.Value, (int)nudR.Value * 2, (int)nudR.Value * 2);
//g.Dispose();
//Dictionary<int, int> spotlabels = new Dictionary<int, int>();
//Dictionary<int, int> spotlabels561 = new Dictionary<int, int>();
//int startX = (int)nudX.Value - (int)nudR.Value - 1;
//startX = startX < 0 ? 0 : startX;
//int endX = (int)nudX.Value + (int)nudR.Value + 1;
//endX = endX > image.Width ? image.Width : endX;
//int startY = (int)nudY.Value - (int)nudR.Value - 1;
//startY = startY < 0 ? 0 : startY;
//int endY = (int)nudY.Value + (int)nudR.Value + 1;
//endY = endY > image.Height ? image.Height : endY;
//for (int x = startX; x < endX; x++)
//{
// for (int y = startY; y < endY; y++)
// {
// int label = components.Labels[x, y];
// Color color = imageEllipse.GetPixel(x, y);
// if (label <= 0 || (color.R == 0 && color.G == 0 && color.B == 0))
// {
// continue;
// }
// if (spotlabels.ContainsKey(label))
// {
// spotlabels[label]++;
// }
// else
// {
// spotlabels[label] = 1;
// }
// }
//}
//for (int x = startX; x < endX; x++)
//{
// for (int y = startY; y < endY; y++)
// {
// int label = components561.Labels[x, y];
// Color color = imageEllipse.GetPixel(x, y);
// if (label <= 0 || (color.R == 0 && color.G == 0 && color.B == 0))
// {
// continue;
// }
// if (spotlabels561.ContainsKey(label))
// {
// spotlabels561[label]++;
// }
// else
// {
// spotlabels561[label] = 1;
// }
// }
//}
//nudCount473.Value = spotlabels.Count;
//nudCount561.Value = spotlabels561.Count;
////**************************************************
this._SetCurrentAnalyzeArea();
return(newSource);
}
