Ejemplo n.º 1
0
        /// <summary>
        /// Returns standard feature class instance, if the key in the specified feature item is found
        /// in the map; otherwise returns the specified feature item itself.
        /// For example:
        /// If the specified feature item has the key "Gene" then this method returns instance of the Gene class
        /// with data copied from the specified item.
        /// </summary>
        /// <param name="item">Feature item instance to which the standard feature item instance is needed.</param>
        /// <returns>If found returns appropriate class instance for the specified feature item, otherwise returns
        /// the specified item itself.</returns>
        public static FeatureItem GetStandardFeatureItem(FeatureItem item)
        {
            Type type = null;

            if (featureMap.ContainsKey(item.Key))
            {
                type = featureMap[item.Key];
            }

            if (type != null)
            {
                FeatureItem newItem = (FeatureItem)Activator.CreateInstance(type, item.Location);

                foreach (KeyValuePair <string, List <string> > kvp in item.Qualifiers)
                {
                    newItem.Qualifiers.Add(kvp.Key, kvp.Value);
                }

                item = newItem;
            }

            return(item);
        }
Ejemplo n.º 2
0
        private void ParseFeatures(MBFTextReader mbfReader, ref Sequence sequence)
        {
            ILocationBuilder locBuilder = LocationBuilder;

            if (locBuilder == null)
            {
                throw new InvalidOperationException(Resource.NullLocationBuild);
            }

            // set data indent for features
            mbfReader.DataIndent = _featureDataIndent;

            // The sub-items of a feature are referred to as qualifiers.  These do not have unique
            // keys, so they are stored as lists in the SubItems dictionary.
            SequenceFeatures    features    = new SequenceFeatures();
            IList <FeatureItem> featureList = features.All;

            while (mbfReader.HasLines)
            {
                if (String.IsNullOrEmpty(mbfReader.Line) || mbfReader.LineHeader == "FEATURES")
                {
                    mbfReader.GoToNextLine();
                    continue;
                }

                if (mbfReader.Line[0] != ' ')
                {
                    // start of non-feature text
                    break;
                }

                if (!mbfReader.LineHasHeader)
                {
                    string message = Properties.Resource.GenbankEmptyFeature;
                    Trace.Report(message);
                    throw new InvalidDataException(message);
                }

                // check for multi-line location string
                string featureKey = mbfReader.LineHeader;
                string location   = mbfReader.LineData;
                mbfReader.GoToNextLine();
                while (mbfReader.HasLines && !mbfReader.LineHasHeader &&
                       mbfReader.LineHasData && !mbfReader.LineData.StartsWith("/", StringComparison.Ordinal))
                {
                    location += mbfReader.LineData;
                    mbfReader.GoToNextLine();
                }

                // create features as MetadataListItems
                FeatureItem feature = new FeatureItem(featureKey, locBuilder.GetLocation(location));

                // process the list of qualifiers, which are each in the form of
                // /key="value"
                string qualifierKey   = string.Empty;
                string qualifierValue = string.Empty;
                while (mbfReader.HasLines)
                {
                    if (!mbfReader.LineHasHeader && mbfReader.LineHasData)
                    {
                        // '/' denotes a continuation of the previous line
                        if (mbfReader.LineData.StartsWith("/", StringComparison.Ordinal))
                        {
                            // new qualifier; save previous if this isn't the first
                            if (!String.IsNullOrEmpty(qualifierKey))
                            {
                                AddQualifierToFeature(feature, qualifierKey, qualifierValue);
                            }

                            // set the key and value of this qualifier
                            int equalsIndex = mbfReader.LineData.IndexOf('=');
                            if (equalsIndex < 0)
                            {
                                // no value, just key (this is allowed, see NC_005213.gbk)
                                qualifierKey   = mbfReader.LineData.Substring(1);
                                qualifierValue = string.Empty;
                            }
                            else if (equalsIndex > 0)
                            {
                                qualifierKey   = mbfReader.LineData.Substring(1, equalsIndex - 1);
                                qualifierValue = mbfReader.LineData.Substring(equalsIndex + 1);
                            }
                            else
                            {
                                string message = String.Format(
                                    CultureInfo.CurrentCulture,
                                    Properties.Resource.GenbankInvalidFeature,
                                    mbfReader.Line);
                                Trace.Report(message);
                                throw new InvalidDataException(message);
                            }
                        }
                        else
                        {
                            // Continuation of previous line; "note" gets a line break, and
                            // everything else except "translation" and "transl_except" gets a
                            // space to separate words.
                            if (qualifierKey == "note")
                            {
                                qualifierValue += Environment.NewLine;
                            }
                            else if (qualifierKey != "translation" && qualifierKey != "transl_except")
                            {
                                qualifierValue += " ";
                            }

                            qualifierValue += mbfReader.LineData;
                        }

                        mbfReader.GoToNextLine();
                    }
                    else if (mbfReader.Line.StartsWith("\t", StringComparison.Ordinal))
                    {
                        // this seems to be data corruption; but BioPerl test set includes
                        // (old, 2003) NT_021877.gbk which has this problem, so we
                        // handle it
                        ApplicationLog.WriteLine("WARN: nonstandard line format at line {0}: '{1}'",
                                                 mbfReader.LineNumber, mbfReader.Line);
                        qualifierValue += " " + mbfReader.Line.Trim();
                        mbfReader.GoToNextLine();
                    }
                    else
                    {
                        break;
                    }
                }

                // add last qualifier
                if (!String.IsNullOrEmpty(qualifierKey))
                {
                    AddQualifierToFeature(feature, qualifierKey, qualifierValue);
                }

                // still add feature, even if it has no qualifiers
                featureList.Add(StandardFeatureMap.GetStandardFeatureItem(feature));
            }

            if (featureList.Count > 0)
            {
                ((GenBankMetadata)sequence.Metadata[Helper.GenBankMetadataKey]).Features = features;
            }
        }