/// <summary>
/// Extraxct wsi in full size. [Might possibly fail due to memory access violations]
/// </summary>
/// <param name="filename">filename of wsi</param>
/// <returns>image as bitmap</returns>
public static Bitmap ExtractImageFromWSIFullSize(string filename)
{
using (VMscope.InteropCore.ImageStreaming.IStreamingImage image = VMscope.VirtualSlideAccess.Sdk.GetImage(filename))
{
Console.WriteLine("Width: " + image.Size.Height + " | Width: " + image.Size.Width + " | Level: " + image.Levels);
Bitmap bmp1 = image.GetImagePart(0, 0, image.Size.Width, image.Size.Height, image.Size.Width / 2, image.Size.Height / 2);
//Bitmap bmp1 = image.GetGlassSlideImage();
Console.WriteLine("Width: " + bmp1.Height + " | Width: " + bmp1.Width);
return(bmp1);
}
}
/// <summary>
/// Extract image from wsi with given resolution level.
/// </summary>
/// <param name="filename">wsi filename</param>
/// <param name="resolution">resolution level (2^level)</param>
/// <returns>whole wsi image downsampled</returns>
public static Image <Bgr, byte> ExtractImageFromWSI(string filename, int resolution)
{
using (VMscope.InteropCore.ImageStreaming.IStreamingImage image = VMscope.VirtualSlideAccess.Sdk.GetImage(filename))
{
int layerFactor = (int)(Math.Pow(2.0, (double)resolution));
Bitmap bmp = image.GetImagePart(0, 0, image.Size.Width, image.Size.Height, (int)(image.Size.Width / layerFactor), (int)(image.Size.Height / layerFactor));
Image <Bgr, byte> m = new Image <Bgr, byte>(bmp);
bmp.Dispose();
return(m);
}
}
private string SaveROIAsPNG(WSIHistoObject histoObject, int resolution)
{
string path = null;
Cursor.Current = Cursors.WaitCursor;
try
{
using (VMscope.InteropCore.ImageStreaming.IStreamingImage image = VMscope.VirtualSlideAccess.Sdk.GetImage(histoObject.wsiImagePath))
{
int layerFactor = (int)(Math.Pow(2.0, resolution));
// is bitmap loaded correctly?
using (Bitmap bmp = image.GetImagePart(histoObject.objRectangle.X, histoObject.objRectangle.Y, histoObject.objRectangle.Width, histoObject.objRectangle.Height, (int)(histoObject.objRectangle.Width / layerFactor), (int)(histoObject.objRectangle.Height / layerFactor)))
{
/*using(BitmapViewer viewer = new BitmapViewer(bmp))
* {
* viewer.ShowDialog();
* }*/
// flip image so it fits the thumbnail
for (int k = 0; k < histoObject.NrOfRotations % 4; k++)
{
bmp.RotateFlip(RotateFlipType.Rotate90FlipNone);
}
if (histoObject.HorizontalFlip)
{
bmp.RotateFlip(RotateFlipType.RotateNoneFlipX);
}
// write image to file
path = Path.Combine(ApplicationContext.OutputPath, textBox1.Text, (histoObject.labelTitle.Text + ".png"));
bmp.Save(path, ImageFormat.Png);
Console.WriteLine("saved image " + histoObject.labelTitle.Text);
bmp.Dispose();
}
image.Dispose();
}
return(path);
} catch (Exception ex)
{
Console.WriteLine(ex);
MessageBox.Show(ex.Message);
return(path);
} finally
{
Cursor.Current = Cursors.Default;
}
}
private void treeView1_NodeMouseClick(object sender, TreeNodeMouseClickEventArgs e)
{
string path = e.Node.Name;
if (path != null)
{
using (VMscope.InteropCore.ImageStreaming.IStreamingImage image = VMscope.VirtualSlideAccess.Sdk.GetImage(path))
{
double ratioWidth = (double)image.Size.Width / (double)image.Size.Height;
double ratioHeight = (double)image.Size.Height / (double)image.Size.Width;
int newWidth = image.Size.Width > image.Size.Height ? pictureBox1.Width : (int)(ratioWidth * pictureBox1.Width);
int newHeight = image.Size.Width > image.Size.Height ? (int)(ratioHeight * pictureBox1.Height) : pictureBox1.Height;
Bitmap bmp = image.GetImagePart(0, 0, image.Size.Width, image.Size.Height, newWidth, newHeight);
pictureBox1.Image = bmp;
}
}
}