private void GetStatisticsROIs()
{
if (imgDrawer == null || imgDrawer.frames == null || fi == null)
{
return;
}
//Generate data table
DataTable tbl = new DataTable();
tbl.Columns.Add(new DataColumn("Coefficient", typeof(string)));
DataRow row = tbl.NewRow();
row["Coefficient"] = "PCC";
tbl.Rows.Add(row);
row = tbl.NewRow();
row["Coefficient"] = "Rsquared";
tbl.Rows.Add(row);
row = tbl.NewRow();
row["Coefficient"] = "MOC";
tbl.Rows.Add(row);
row = tbl.NewRow();
row["Coefficient"] = "MCC 1";
tbl.Rows.Add(row);
row = tbl.NewRow();
row["Coefficient"] = "MCC 2";
tbl.Rows.Add(row);
//Points list
Point[] points = null;
ROI roi = null;
Cell_Tool_3.FrameCalculator frameCalc = new Cell_Tool_3.FrameCalculator();
int[] curFrames = new int[2];
fi.frame = imgDrawer.frames[0];
int roiFrame = frameCalc.FrameC(fi, 0);
curFrames[0] = frameCalc.FrameC(fi, imgDrawer.colIndexes[0]);
fi.frame = imgDrawer.frames[1];
curFrames[1] = frameCalc.FrameC(fi, imgDrawer.colIndexes[1]);
if (fi.roiList != null && fi.roiList.Length > 0 && fi.roiList[0] != null)
{
for (int i = 0; i < fi.roiList[0].Count; i++)
{
roi = fi.roiList[0][i];
points = ROIManager.GetROIPoints(roiFrame, roi, fi).ToArray();
if (points == null || points.Length < 2)
{
return;
}
points = CheckArePointsInTheImage(points, fi.sizeX, fi.sizeY);
if (points == null || points.Length < 2)
{
return;
}
switch (fi.bitsPerPixel)
{
case 8:
points = Operations.PairImages(fi.image8bit[curFrames[0]], fi.image8bit[curFrames[1]], points);
break;
case 16:
points = Operations.PairImages(fi.image16bit[curFrames[0]], fi.image16bit[curFrames[1]], points);
break;
}
if (points == null || points.Length < 2)
{
return;
}
double[] MCC = null;
//Filter By thresholds
if (imgDrawer.showBinary)
{
points = Operations.FilterPointsByThresholds(points, imgDrawer.thresholds[0], imgDrawer.thresholds[1]);
// MessageBox.Show(points.Length.ToString());
MCC = Operations.MCC(points, imgDrawer.thresholds[0], imgDrawer.thresholds[1]);
}
else
{
MCC = Operations.MCC(points, 1, 1);
}
//processing
double PCC = Operations.PCC(points);
double Rsquare = Math.Pow(PCC, 2.0);
double MOC = Operations.MOC(points);
tbl.Columns.Add(new DataColumn("ROI " + (i + 1), typeof(double)));
row = tbl.Rows[0];
row["ROI " + (i + 1)] = PCC;
row = tbl.Rows[1];
row["ROI " + (i + 1)] = Rsquare;
row = tbl.Rows[2];
row["ROI " + (i + 1)] = MOC;
row = tbl.Rows[3];
row["ROI " + (i + 1)] = MCC[0];
row = tbl.Rows[4];
row["ROI " + (i + 1)] = MCC[1];
}
}
dataGridView1.DataSource = tbl;
}
private void button_SaveResults_Click(object sender, EventArgs e)
{
try
{
//get names
string str = "";
string dir = fi.Dir;
string name = "(1)";
dir = dir.Substring(0, dir.LastIndexOf("."));
name = dir.Substring(dir.LastIndexOf("\\") + 1, dir.Length - dir.LastIndexOf("\\") - 1);
if (!Directory.Exists(dir))
{
Directory.CreateDirectory(dir);
}
//export settings
str = "File:\t" + dir;
str += "\nCh1:\t" + imgDrawer.colIndexes[0];
str += "\nCh2:\t" + imgDrawer.colIndexes[1];
str += "\nLock frames:\t" + checkBox_LockFrames.Checked;
str += "\nTime Frame Ch1:\t" + trackBar_TimeCh1.Value;
str += "\nTime Frame Ch2:\t" + trackBar_TimeCh2.Value;
str += "\nUse thresholds:\t" + checkBox_UseBinary.Checked;
str += "\nThreshold Ch1:\t" + trackBar_ThreshCh1.Value;
str += "\nThreshold Ch2:\t" + trackBar_ThreshCh2.Value;
str += "\nUse ROIs:\t" + checkBox_UseROIs.Checked;
str += "\n\nResults:\n";
//export dataTable
using (StreamWriter write = new StreamWriter(File.Create(dir + @"\" + name + "_DataTable.txt")))
{
int row = dataGridView1.Rows.Count;
int cell = dataGridView1.Rows[1].Cells.Count;
for (int i = 0; i < dataGridView1.Columns.Count; i++)
{
str += dataGridView1.Columns[i].Name + "\t";
}
write.WriteLine(str);
for (int i = 0; i < row; i++)
{
str = "";
for (int j = 0; j < cell; j++)
{
if (dataGridView1.Rows[i].Cells[j].Value == null)
{
dataGridView1.Rows[i].Cells[j].Value = "null";
}
str += dataGridView1.Rows[i].Cells[j].Value.ToString() + "\t";
}
write.WriteLine(str);
}
write.Close();
write.Dispose();
}
//Export plots
Point[] values = null;
List <Point> points = new List <Point>();
List <Point> pointsFilt = new List <Point>();
List <Point> valuesFilt = new List <Point>();
Cell_Tool_3.FrameCalculator frameCalc = new Cell_Tool_3.FrameCalculator();
int[] curFrames = new int[2];
fi.frame = imgDrawer.frames[0];
int roiFrame = frameCalc.FrameC(fi, 0);
curFrames[0] = frameCalc.FrameC(fi, imgDrawer.colIndexes[0]);
fi.frame = imgDrawer.frames[1];
curFrames[1] = frameCalc.FrameC(fi, imgDrawer.colIndexes[1]);
{
//Whole image
switch (fi.bitsPerPixel)
{
case 8:
values = Operations.PairImages(fi.image8bit[curFrames[0]], fi.image8bit[curFrames[1]]);
break;
case 16:
values = Operations.PairImages(fi.image16bit[curFrames[0]], fi.image16bit[curFrames[1]]);
break;
}
for (int y = 0, i = 0; y < fi.sizeY; y++)
{
for (int x = 0; x < fi.sizeX; x++, i++)
{
points.Add(new Point(x, y));
if (values[i].X >= imgDrawer.thresholds[0] || values[i].Y >= imgDrawer.thresholds[1])
{
pointsFilt.Add(points[i]);
valuesFilt.Add(values[i]);
}
}
}
ExportScaterPlot(dir, name + "_All", values, points.ToArray());
if (checkBox_UseBinary.Checked)
{
ExportScaterPlot(dir, name + "_All_noBG", valuesFilt.ToArray(), pointsFilt.ToArray());
}
points.Clear();
pointsFilt.Clear();
valuesFilt.Clear();
}
if (checkBox_UseROIs.Checked && fi.roiList != null && fi.roiList.Length > 0 && fi.roiList[0] != null)
{
for (int n = 0; n < fi.roiList[0].Count; n++)
{
ROI roi = fi.roiList[0][n];
points = ROIManager.GetROIPoints(roiFrame, roi, fi);
points = CheckArePointsInTheImage(points.ToArray(), fi.sizeX, fi.sizeY).ToList();
//Whole image
switch (fi.bitsPerPixel)
{
case 8:
values = Operations.PairImages(fi.image8bit[curFrames[0]], fi.image8bit[curFrames[1]], points.ToArray());
break;
case 16:
values = Operations.PairImages(fi.image16bit[curFrames[0]], fi.image16bit[curFrames[1]], points.ToArray());
break;
}
for (int i = 0; i < points.Count; i++)
{
if (values[i].X >= imgDrawer.thresholds[0] || values[i].Y >= imgDrawer.thresholds[1])
{
pointsFilt.Add(points[i]);
valuesFilt.Add(values[i]);
}
}
ExportScaterPlot(dir, name + "_ROI" + (n + 1), values, points.ToArray());
if (checkBox_UseBinary.Checked)
{
ExportScaterPlot(dir, name + "_ROI" + (n + 1) + "_noBG", valuesFilt.ToArray(), pointsFilt.ToArray());
}
points.Clear();
pointsFilt.Clear();
valuesFilt.Clear();
}
}
//exit
MessageBox.Show("Files saved!");
}
catch
{
MessageBox.Show("Error: Files NOT saved!");
}
}
private void GetStatistics()
{
if (checkBox_UseROIs.Checked && checkBox_UseROIs.Enabled)
{
GetStatisticsROIs();
return;
}
if (imgDrawer == null || imgDrawer.frames == null || fi == null)
{
return;
}
Point[] points = null;
Cell_Tool_3.FrameCalculator frameCalc = new Cell_Tool_3.FrameCalculator();
int[] curFrames = new int[2];
fi.frame = imgDrawer.frames[0];
curFrames[0] = frameCalc.FrameC(fi, imgDrawer.colIndexes[0]);
fi.frame = imgDrawer.frames[1];
curFrames[1] = frameCalc.FrameC(fi, imgDrawer.colIndexes[1]);
switch (fi.bitsPerPixel)
{
case 8:
points = Operations.PairImages(fi.image8bit[curFrames[0]], fi.image8bit[curFrames[1]]);
break;
case 16:
points = Operations.PairImages(fi.image16bit[curFrames[0]], fi.image16bit[curFrames[1]]);
break;
}
if (points == null)
{
return;
}
double[] MCC = null;
//Filter By thresholds
if (imgDrawer.showBinary)
{
points = Operations.FilterPointsByThresholds(points, imgDrawer.thresholds[0], imgDrawer.thresholds[1]);
MCC = Operations.MCC(points, imgDrawer.thresholds[0], imgDrawer.thresholds[1]);
}
else
{
MCC = Operations.MCC(points, 1, 1);
}
//processing
double PCC = Operations.PCC(points);
double Rsquare = Math.Pow(PCC, 2.0);
double MOC = Operations.MOC(points);
// MessageBox.Show("PCC: " + PCC + "\nRsquared: " + Rsquare+"\nMOC: " + MOC
// + "\nMCC 1: " + MCC[0] + "\nMCC 2: " + MCC[1]);
//Generate data table
DataTable tbl = new DataTable();
tbl.Columns.Add(new DataColumn("Coefficient", typeof(string)));
tbl.Columns.Add(new DataColumn("Value", typeof(double)));
DataRow row = tbl.NewRow();
row["Coefficient"] = "PCC";
row["Value"] = PCC;
tbl.Rows.Add(row);
row = tbl.NewRow();
row["Coefficient"] = "Rsquared";
row["Value"] = Rsquare;
tbl.Rows.Add(row);
row = tbl.NewRow();
row["Coefficient"] = "MOC";
row["Value"] = MOC;
tbl.Rows.Add(row);
row = tbl.NewRow();
row["Coefficient"] = "MCC 1";
row["Value"] = MCC[0];
tbl.Rows.Add(row);
row = tbl.NewRow();
row["Coefficient"] = "MCC 2";
row["Value"] = MCC[1];
tbl.Rows.Add(row);
dataGridView1.DataSource = tbl;
}