public async Task <bool> AutostartAnalysis(GNAnalysisRequest analysis)
{
AnalysisRequestService analysisService = new AnalysisRequestService(db);
GNContact userContact = db.GNContacts.Find(Guid.Parse("0750f896-e7d6-48d4-a1b9-007059f62784"));
await analysisService.StartAnalysis(userContact, analysis.Id);
return(true);
}
private static void ReStartFailedAnalysis(AnalysisRequestService analysisRequestService, GNAnalysisRequest analysisRequest)
{
if (RESTART_ANALYSIS_FEATURE_ACTIVE)
{
try
{
//get analysis request from db
GNAnalysisRequest ar = analysisRequestService.db.GNAnalysisRequests
.Include(a => a.AnalysisType)
.Include(a => a.GNAnalysisRequestGNSamples.Select(s => s.GNSample).Select(s => s.CloudFiles))
.Include(a => a.AnalysisStatus)
.Include(a => a.AnalysisResult)
.Where(a => a.Id == analysisRequest.Id)
.FirstOrDefault();
//determine if analysis re-start is allowed
ar = analysisRequestService.IsValidSampleSet(ar);
ar.CanReStartAnalysis = analysisRequestService.IsAnalysisRestartAllowed(ar, FORCE_RESTART_ANALYSIS);
//restart analysis
if (ar.CanReStartAnalysis && ar.IsFailedRequest)
{
LogUtil.Info(logger, "Restart Analysis for AR ID [" + ar.Id + "]...");
System.Console.WriteLine("\nRestart Analysis for AR ID [" + ar.Id + "]...");
var t = Task.Run(async delegate
{
var arService = new AnalysisRequestService(new GNEntityModelContainer(), new IdentityModelContainer());
//get user contact
GNContact userContact = arService.db.GNContacts.Find(ar.CreatedBy);
if (arService.analysisRequestPendingCloudMessageService == null || arService.cloudStorageService == null)
{
arService.InitCloudServices(userContact.GNOrganization.AWSConfigId);
}
await arService.StartAnalysis(userContact, ar.Id);
});
}
else
{
LogUtil.Info(logger, "UNABLE to Restart Analysis for AR ID [" + ar.Id + "]...");
System.Console.WriteLine("\nUNABLE to Restart Analysis for AR ID [" + ar.Id + "]...");
}
}
catch (Exception e)
{
LogUtil.Error(logger, "Unable to Process Failure of Analysis " + analysisRequest.Id + " : " + e.Message, e);
}
}
}
public async Task <bool> ProcessQCMessage(NewSampleBatchStatus newSampleMessageStatus)
{
System.Console.WriteLine("QCQCQC " + newSampleMessageStatus.name);
try
{
GNCloudFileCategory fileCategory = db.GNCloudFileCategories.Where(a => a.Id == 1).FirstOrDefault();
string sampleName1 = newSampleMessageStatus.name;
string sampleName = sampleName1.Substring(0, sampleName1.IndexOf("_"));
//s3://sanford-fastq/151204_SN7001383_0164_AC7Y91ACXX/FASTQ/fastqc/AAA076960/AAA076960_S6_R2_001/
GNSample sample = db.GNSamples.Where(a => a.Name.Equals(sampleName) && a.IsReady == false).FirstOrDefault();
GNCloudStorageService st = new GNCloudStorageService();
string volume = "dev-gn-s3-01";
Guid fileId = Guid.NewGuid();
string folderPath = "fastq/" + sample.Id + "/" + fileId + "/";
string fastqFileName = folderPath + newSampleMessageStatus.name + ".fastq.gz";
string fileURL = "https://dev-gn-s3-01.s3.amazonaws.com/" + fastqFileName;
string originBucket = newSampleMessageStatus.filesBucket;
originBucket = originBucket.Replace("s3://", "");
originBucket = originBucket.Substring(0, originBucket.IndexOf("/")); //"sanford-fastq";
string QcOriginKey = newSampleMessageStatus.filesBucket;
QcOriginKey = QcOriginKey.Replace("s3://", "");
QcOriginKey = QcOriginKey.Substring(originBucket.Length + 1);
string FileDescription = newSampleMessageStatus.name + ".fastq.gz";
string fastqOriginKey = QcOriginKey.Substring(0, QcOriginKey.IndexOf("FASTQ/") + 5);
fastqOriginKey = fastqOriginKey + "/" + FileDescription;
QcOriginKey = QcOriginKey + "fastqc_report.html";
string destinationBucket = "dev-gn-s3-01/" + folderPath;
destinationBucket = "dev-gn-s3-01";
string QcFileName = folderPath + "fastqc_report.html";
string QcDestinationURL = "https://dev-gn-s3-01.s3.amazonaws.com/" + QcFileName;
st.CopyS3Object(originBucket, fastqOriginKey, destinationBucket, fastqFileName);
st.CopyS3Object(originBucket, QcOriginKey, destinationBucket, QcFileName);
GNCloudFile cloudFile = new GNCloudFile
{
Id = fileId,
GNCloudFileCategoryId = fileCategory.Id, //FASTQ
CloudFileCategory = fileCategory,
FileName = folderPath + fastqFileName,
FileURL = fileURL,
FolderPath = folderPath,
Volume = volume,
Description = fastqFileName,
FileSize = st.GetObjectSize(originBucket, fastqOriginKey),
AWSRegionSystemName = db.AWSRegions.FirstOrDefault().AWSRegionSystemName,
CreateDateTime = DateTime.Now,
CreatedBy = Guid.Parse("0750f896-e7d6-48d4-a1b9-007059f62784"),
SampleId = sample.Id.ToString(),
PassedQC = (newSampleMessageStatus.isError.Equals("false")),
QcReportLocation = QcDestinationURL
};
db.GNCloudFiles.Add(cloudFile);
sample.CloudFiles.Add(cloudFile);
if (sample.CloudFiles.Count() == 2 && sample.CloudFiles.Where(a => a.PassedQC == false).Count() == 0)
{
sample.IsReady = true;
if (sample.GNNewSampleBatchSample.GNNewSampleBatch.AutoStartAnalysis)
{
GNAnalysisRequest analysis = sample.GNAnalysisRequestGNSamples.FirstOrDefault().GNAnalysisRequest;
AnalysisRequestService analysisService = new AnalysisRequestService(db);
GNContact userContact = db.GNContacts.Find(Guid.Parse("0750f896-e7d6-48d4-a1b9-007059f62784"));
await analysisService.StartAnalysis(userContact, analysis.Id);
}
}
db.SaveChanges();
}
catch (Exception e)
{
System.Console.WriteLine("***Unable to Update QC-COMPLETED status. " + e.Message + e.StackTrace + " **********************************");
Exception e2 = new Exception("Unable to Update QC-COMPLETED status.", e);
LogUtil.Warn(logger, e2.Message, e2);
return(false);
}
return(true);
}