private string checkPCRResult(PCRColumnData data, string testItemName, bool isSingle, Tubetype tubeType)
{
string rox = null;
string hex = null;
string fam = null;
if (data is ABIPCRColumnData)
{
ABIPCRColumnData aiData = (ABIPCRColumnData)data;
if ("ROX" == aiData.Detector)
{
rox = aiData.Ct;
}
else if ("FAM" == aiData.Detector)
{
fam = aiData.Ct;
}
else if ("HEX" == aiData.Detector)
{
hex = aiData.Ct;
}
if (aiData.DataList != null)
{
foreach (ABIPCRColumnData subAiData in aiData.DataList)
{
if ("ROX" == subAiData.Detector)
{
rox = subAiData.Ct;
}
else if ("FAM" == subAiData.Detector)
{
fam = subAiData.Ct;
}
else if ("HEX" == subAiData.Detector)
{
hex = subAiData.Ct;
}
}
}
}
else if (data is BIORADPCRColumnData)
{
BIORADPCRColumnData bioData = (BIORADPCRColumnData)data;
if ("FAM" == bioData.Fluor)
{
fam = bioData.Cq;
}
else if ("HEX" == bioData.Fluor)
{
hex = bioData.Cq;
}
else if ("ROX" == bioData.Fluor)
{
rox = bioData.Cq;
}
if (bioData.DataList != null)
{
foreach (BIORADPCRColumnData subBioData in bioData.DataList)
{
if ("FAM" == subBioData.Fluor)
{
fam = subBioData.Cq;
}
else if ("HEX" == subBioData.Fluor)
{
hex = subBioData.Cq;
}
else if ("ROX" == subBioData.Fluor)
{
rox = subBioData.Cq;
}
}
}
}
else if (data is StratagenePCRColumnData)
{
StratagenePCRColumnData sData = (StratagenePCRColumnData)data;
if ("FAM" == sData.Dye)
{
fam = sData.Ct;
}
else if ("HEX" == sData.Dye)
{
hex = sData.Ct;
}
else if ("ROX" == sData.Dye)
{
rox = sData.Ct;
}
if (sData.DataList != null)
{
foreach (StratagenePCRColumnData subSata in sData.DataList)
{
if ("FAM" == subSata.Dye)
{
fam = subSata.Ct;
}
else if ("HEX" == subSata.Dye)
{
hex = subSata.Ct;
}
else if ("ROX" == subSata.Dye)
{
rox = subSata.Ct;
}
}
}
}
if (!string.IsNullOrEmpty(fam) && (fam == "Undetermined" || fam == "No Ct" || fam == "N/A"))
{
fam = "45";
}
if (!string.IsNullOrEmpty(hex) && (hex == "Undetermined" || hex == "No Ct" || hex == "N/A"))
{
hex = "45";
}
if (!string.IsNullOrEmpty(rox) && (rox == "Undetermined" || rox == "No Ct" || rox == "N/A"))
{
rox = "45";
}
float outNumber = 0;
//阴性对照
if (tubeType == Tubetype.NegativeControl)
{
if (testItemName == "HBV")
{
if (!string.IsNullOrEmpty(hex) && float.TryParse(hex, out outNumber))
{
float hexNumber = float.Parse(hex);
if (hexNumber > 37 && !string.IsNullOrEmpty(rox) && float.TryParse(rox, out outNumber) && float.Parse(rox) < 36)
{
return(PCRTest.NegativeResult);
}
}
return(PCRTest.InvalidResult);
}
else if (testItemName == "HCV")
{
if (!string.IsNullOrEmpty(fam) && float.TryParse(fam, out outNumber))
{
float famNumber = float.Parse(fam);
if (famNumber > 42 && !string.IsNullOrEmpty(rox) && float.TryParse(rox, out outNumber) && float.Parse(rox) < 36)
{
return(PCRTest.NegativeResult);
}
}
return(PCRTest.InvalidResult);
}
else if (testItemName == "HIV")
{
if (!string.IsNullOrEmpty(fam) && float.TryParse(fam, out outNumber))
{
float famNumber = float.Parse(fam);
if (famNumber > 42 && !string.IsNullOrEmpty(rox) && float.TryParse(rox, out outNumber) && float.Parse(rox) < 36)
{
return(PCRTest.NegativeResult);
}
}
return(PCRTest.InvalidResult);
}
else
{
return(PCRTest.NoResult);
}
}
else if (tubeType == Tubetype.PositiveControl)
{
if (testItemName == "HBV")
{
if (!string.IsNullOrEmpty(hex) && float.TryParse(hex, out outNumber))
{
float hexNumber = float.Parse(hex);
if (hexNumber <= 33 && !string.IsNullOrEmpty(rox) && float.TryParse(rox, out outNumber) && float.Parse(rox) < 36)
{
return(PCRTest.PositiveResult);
}
}
return(PCRTest.InvalidResult);
}
else if (testItemName == "HCV")
{
if (!string.IsNullOrEmpty(fam) && float.TryParse(fam, out outNumber))
{
float famNumber = float.Parse(fam);
if (famNumber <= 37 && !string.IsNullOrEmpty(rox) && float.TryParse(rox, out outNumber) && float.Parse(rox) < 36)
{
return(PCRTest.PositiveResult);
}
}
return(PCRTest.InvalidResult);
}
else if (testItemName == "HIV")
{
if (!string.IsNullOrEmpty(fam) && float.TryParse(fam, out outNumber))
{
float famNumber = float.Parse(fam);
if (famNumber <= 37 && !string.IsNullOrEmpty(rox) && float.TryParse(rox, out outNumber) && float.Parse(rox) < 36)
{
return(PCRTest.PositiveResult);
}
}
return(PCRTest.InvalidResult);
}
else
{
return(PCRTest.NoResult);
}
}
float HBVValue = 0;
float HCVValue = 0;
float HIVValue = 0;
if (isSingle)
{
HBVValue = 35;
HCVValue = 42;
HIVValue = 40;
}
else
{
HBVValue = 37;
HCVValue = 42;
HIVValue = 42;
}
if (testItemName == "HBV")
{
if (!string.IsNullOrEmpty(hex) && float.TryParse(hex, out outNumber))
{
float hexNumber = float.Parse(hex);
if (hexNumber > HBVValue && !string.IsNullOrEmpty(rox) && float.TryParse(rox, out outNumber) && float.Parse(rox) < 36)
{
return(PCRTest.NegativeResult);
}
else if (hexNumber <= HBVValue)
{
return(PCRTest.PositiveResult);
}
else if (hexNumber > HBVValue && !string.IsNullOrEmpty(rox) && float.TryParse(rox, out outNumber) && float.Parse(rox) >= 36)
{
return(PCRTest.InvalidResult);
}
else
{
return(PCRTest.NoResult);
}
}
else
{
return(PCRTest.NoResult);
}
}
else if (testItemName == "HCV")
{
if (!string.IsNullOrEmpty(fam) && float.TryParse(fam, out outNumber))
{
float famNumber = float.Parse(fam);
if (famNumber > HCVValue && !string.IsNullOrEmpty(rox) && float.TryParse(rox, out outNumber) && float.Parse(rox) < 36)
{
return(PCRTest.NegativeResult);
}
else if (famNumber <= HCVValue)
{
return(PCRTest.PositiveResult);
}
else if (famNumber > HCVValue && !string.IsNullOrEmpty(rox) && float.TryParse(rox, out outNumber) && float.Parse(rox) >= 36)
{
return(PCRTest.InvalidResult);
}
else
{
return(PCRTest.NoResult);
}
}
else
{
return(PCRTest.NoResult);
}
}
else if (testItemName == "HIV")
{
if (!string.IsNullOrEmpty(fam) && float.TryParse(fam, out outNumber))
{
float famNumber = float.Parse(fam);
if (famNumber > HIVValue && !string.IsNullOrEmpty(rox) && float.TryParse(rox, out outNumber) && float.Parse(rox) < 36)
{
return(PCRTest.NegativeResult);
}
else if (famNumber <= HIVValue)
{
return(PCRTest.PositiveResult);
}
else if (famNumber > HIVValue && !string.IsNullOrEmpty(rox) && float.TryParse(rox, out outNumber) && float.Parse(rox) >= 36)
{
return(PCRTest.InvalidResult);
}
else
{
return(PCRTest.NoResult);
}
}
else
{
return(PCRTest.NoResult);
}
}
else
{
return(PCRTest.NoResult);
}
}
private bool processBIORADFile(string fileName, Guid rotationId, Plate plate, out bool isRightPositionNumber)
{
isRightPositionNumber = true;
string formatTitle = "Well,Fluor,Target,Content,Sample,Cq,Starting Quantity (SQ)";
bool isRightFormat = false;
System.Collections.Generic.Dictionary <string, BIORADPCRColumnData> dataList = new System.Collections.Generic.Dictionary <string, BIORADPCRColumnData>();
using (FileStream stream = File.Open(fileName, FileMode.Open, FileAccess.Read))
{
using (StreamReader reader = new StreamReader(stream))
{
while (!reader.EndOfStream)
{
string content = reader.ReadLine();
if (isRightFormat)
{
string[] values = content.Split(',');
if (values.Count() < 6)
{
break;
}
BIORADPCRColumnData bioData = new BIORADPCRColumnData();
bioData.Well = values[0];
bioData.Fluor = values[1];
bioData.Cq = values[5];
bioData.XmlContent = "<Well>" + values[0] + "</Well><Fluor>" + values[1] + "</Fluor><Cq>" + values[5] + "</Cq>";
if (!dataList.ContainsKey(bioData.Well))
{
dataList.Add(bioData.Well, bioData);
}
else
{
BIORADPCRColumnData _bioData = (BIORADPCRColumnData)dataList[bioData.Well];
if (_bioData.DataList == null)
{
_bioData.DataList = new List <PCRColumnData>();
_bioData.DataList.Add(bioData);
}
else
{
_bioData.DataList.Add(bioData);
}
}
}
if (!isRightFormat && content.StartsWith(formatTitle))
{
isRightFormat = true;
}
}
}
}
if (!isRightFormat)
{
return(isRightFormat);
}
Dictionary <int, DataRow> dc = controller.GetPCRPositionsByPlateID(plate.PlateID, currentExperimentId);
if (dc.Count != dataList.Count)
{
isRightPositionNumber = false;
if (!isRightPositionNumber)
{
MessageBoxResult selectResult = MessageBox.Show("PCR仪检测结果和混样数不一致，请检查操作是否正确？“是”将按现有规则导入", "系统提示", MessageBoxButton.YesNo);
if (selectResult == MessageBoxResult.No)
{
return(isRightFormat);
}
else
{
isRightPositionNumber = true;
}
}
}
foreach (BIORADPCRColumnData bioData in dataList.Values)
{
PCRTestResult info = new PCRTestResult();
info.ItemID = WanTaiObjectService.NewSequentialGuid();
info.RotationID = rotationId;
info.Position = controller.ChangeCharacterToPositionNumber(bioData.Well);
info.CreateTime = DateTime.Now;
//calculate
if (dc.ContainsKey(info.Position))
{
DataRow row = dc[info.Position];
bool isSingle = (int)row["TubeNumber"] == 1 ? true : false;
Tubetype tubeType = (Tubetype)((short)row["TubeType"]);
info.Result = checkPCRResult(bioData, row["TestName"].ToString(), isSingle, tubeType);
}
info.ExperimentID = currentExperimentId;
if (plate.BarCode != null)
{
info.BarCode = plate.BarCode;
}
info.PlateID = plate.PlateID;
string xmlContent = bioData.XmlContent;
if (bioData.DataList != null && bioData.DataList.Count > 0)
{
foreach (BIORADPCRColumnData data in bioData.DataList)
{
xmlContent = xmlContent + data.XmlContent;
}
}
info.PCRContent = "<PCRContent>" + xmlContent + "</PCRContent>";
pcrResultList.Add(info);
}
return(isRightFormat);
}