private string checkPCRResult(PCRColumnData data, string testItemName, bool isSingle, Tubetype tubeType)
{
string rox = null;
string hex = null;
string fam = null;
if (data is ABIPCRColumnData)
{
ABIPCRColumnData aiData = (ABIPCRColumnData)data;
if ("ROX" == aiData.Detector)
{
rox = aiData.Ct;
}
else if ("FAM" == aiData.Detector)
{
fam = aiData.Ct;
}
else if ("HEX" == aiData.Detector)
{
hex = aiData.Ct;
}
if (aiData.DataList != null)
{
foreach (ABIPCRColumnData subAiData in aiData.DataList)
{
if ("ROX" == subAiData.Detector)
{
rox = subAiData.Ct;
}
else if ("FAM" == subAiData.Detector)
{
fam = subAiData.Ct;
}
else if ("HEX" == subAiData.Detector)
{
hex = subAiData.Ct;
}
}
}
}
else if (data is BIORADPCRColumnData)
{
BIORADPCRColumnData bioData = (BIORADPCRColumnData)data;
if ("FAM" == bioData.Fluor)
{
fam = bioData.Cq;
}
else if ("HEX" == bioData.Fluor)
{
hex = bioData.Cq;
}
else if ("ROX" == bioData.Fluor)
{
rox = bioData.Cq;
}
if (bioData.DataList != null)
{
foreach (BIORADPCRColumnData subBioData in bioData.DataList)
{
if ("FAM" == subBioData.Fluor)
{
fam = subBioData.Cq;
}
else if ("HEX" == subBioData.Fluor)
{
hex = subBioData.Cq;
}
else if ("ROX" == subBioData.Fluor)
{
rox = subBioData.Cq;
}
}
}
}
else if (data is StratagenePCRColumnData)
{
StratagenePCRColumnData sData = (StratagenePCRColumnData)data;
if ("FAM" == sData.Dye)
{
fam = sData.Ct;
}
else if ("HEX" == sData.Dye)
{
hex = sData.Ct;
}
else if ("ROX" == sData.Dye)
{
rox = sData.Ct;
}
if (sData.DataList != null)
{
foreach (StratagenePCRColumnData subSata in sData.DataList)
{
if ("FAM" == subSata.Dye)
{
fam = subSata.Ct;
}
else if ("HEX" == subSata.Dye)
{
hex = subSata.Ct;
}
else if ("ROX" == subSata.Dye)
{
rox = subSata.Ct;
}
}
}
}
if (!string.IsNullOrEmpty(fam) && (fam == "Undetermined" || fam == "No Ct" || fam == "N/A"))
{
fam = "45";
}
if (!string.IsNullOrEmpty(hex) && (hex == "Undetermined" || hex == "No Ct" || hex == "N/A"))
{
hex = "45";
}
if (!string.IsNullOrEmpty(rox) && (rox == "Undetermined" || rox == "No Ct" || rox == "N/A"))
{
rox = "45";
}
float outNumber = 0;
//阴性对照
if (tubeType == Tubetype.NegativeControl)
{
if (testItemName == "HBV")
{
if (!string.IsNullOrEmpty(hex) && float.TryParse(hex, out outNumber))
{
float hexNumber = float.Parse(hex);
if (hexNumber > 37 && !string.IsNullOrEmpty(rox) && float.TryParse(rox, out outNumber) && float.Parse(rox) < 36)
{
return(PCRTest.NegativeResult);
}
}
return(PCRTest.InvalidResult);
}
else if (testItemName == "HCV")
{
if (!string.IsNullOrEmpty(fam) && float.TryParse(fam, out outNumber))
{
float famNumber = float.Parse(fam);
if (famNumber > 42 && !string.IsNullOrEmpty(rox) && float.TryParse(rox, out outNumber) && float.Parse(rox) < 36)
{
return(PCRTest.NegativeResult);
}
}
return(PCRTest.InvalidResult);
}
else if (testItemName == "HIV")
{
if (!string.IsNullOrEmpty(fam) && float.TryParse(fam, out outNumber))
{
float famNumber = float.Parse(fam);
if (famNumber > 42 && !string.IsNullOrEmpty(rox) && float.TryParse(rox, out outNumber) && float.Parse(rox) < 36)
{
return(PCRTest.NegativeResult);
}
}
return(PCRTest.InvalidResult);
}
else
{
return(PCRTest.NoResult);
}
}
else if (tubeType == Tubetype.PositiveControl)
{
if (testItemName == "HBV")
{
if (!string.IsNullOrEmpty(hex) && float.TryParse(hex, out outNumber))
{
float hexNumber = float.Parse(hex);
if (hexNumber <= 33 && !string.IsNullOrEmpty(rox) && float.TryParse(rox, out outNumber) && float.Parse(rox) < 36)
{
return(PCRTest.PositiveResult);
}
}
return(PCRTest.InvalidResult);
}
else if (testItemName == "HCV")
{
if (!string.IsNullOrEmpty(fam) && float.TryParse(fam, out outNumber))
{
float famNumber = float.Parse(fam);
if (famNumber <= 37 && !string.IsNullOrEmpty(rox) && float.TryParse(rox, out outNumber) && float.Parse(rox) < 36)
{
return(PCRTest.PositiveResult);
}
}
return(PCRTest.InvalidResult);
}
else if (testItemName == "HIV")
{
if (!string.IsNullOrEmpty(fam) && float.TryParse(fam, out outNumber))
{
float famNumber = float.Parse(fam);
if (famNumber <= 37 && !string.IsNullOrEmpty(rox) && float.TryParse(rox, out outNumber) && float.Parse(rox) < 36)
{
return(PCRTest.PositiveResult);
}
}
return(PCRTest.InvalidResult);
}
else
{
return(PCRTest.NoResult);
}
}
float HBVValue = 0;
float HCVValue = 0;
float HIVValue = 0;
if (isSingle)
{
HBVValue = 35;
HCVValue = 42;
HIVValue = 40;
}
else
{
HBVValue = 37;
HCVValue = 42;
HIVValue = 42;
}
if (testItemName == "HBV")
{
if (!string.IsNullOrEmpty(hex) && float.TryParse(hex, out outNumber))
{
float hexNumber = float.Parse(hex);
if (hexNumber > HBVValue && !string.IsNullOrEmpty(rox) && float.TryParse(rox, out outNumber) && float.Parse(rox) < 36)
{
return(PCRTest.NegativeResult);
}
else if (hexNumber <= HBVValue)
{
return(PCRTest.PositiveResult);
}
else if (hexNumber > HBVValue && !string.IsNullOrEmpty(rox) && float.TryParse(rox, out outNumber) && float.Parse(rox) >= 36)
{
return(PCRTest.InvalidResult);
}
else
{
return(PCRTest.NoResult);
}
}
else
{
return(PCRTest.NoResult);
}
}
else if (testItemName == "HCV")
{
if (!string.IsNullOrEmpty(fam) && float.TryParse(fam, out outNumber))
{
float famNumber = float.Parse(fam);
if (famNumber > HCVValue && !string.IsNullOrEmpty(rox) && float.TryParse(rox, out outNumber) && float.Parse(rox) < 36)
{
return(PCRTest.NegativeResult);
}
else if (famNumber <= HCVValue)
{
return(PCRTest.PositiveResult);
}
else if (famNumber > HCVValue && !string.IsNullOrEmpty(rox) && float.TryParse(rox, out outNumber) && float.Parse(rox) >= 36)
{
return(PCRTest.InvalidResult);
}
else
{
return(PCRTest.NoResult);
}
}
else
{
return(PCRTest.NoResult);
}
}
else if (testItemName == "HIV")
{
if (!string.IsNullOrEmpty(fam) && float.TryParse(fam, out outNumber))
{
float famNumber = float.Parse(fam);
if (famNumber > HIVValue && !string.IsNullOrEmpty(rox) && float.TryParse(rox, out outNumber) && float.Parse(rox) < 36)
{
return(PCRTest.NegativeResult);
}
else if (famNumber <= HIVValue)
{
return(PCRTest.PositiveResult);
}
else if (famNumber > HIVValue && !string.IsNullOrEmpty(rox) && float.TryParse(rox, out outNumber) && float.Parse(rox) >= 36)
{
return(PCRTest.InvalidResult);
}
else
{
return(PCRTest.NoResult);
}
}
else
{
return(PCRTest.NoResult);
}
}
else
{
return(PCRTest.NoResult);
}
}
private bool processABIFile(string fileName, Guid rotationId, Plate plate, out bool isRightPositionNumber)
{
isRightPositionNumber = true;
string formatTitle = "Well,Sample Name,Detector,Task,Ct,StdDev Ct,Qty,Mean Qty,StdDev Qty,Filtered,Tm";
bool isRightFormat = false;
ABIPCRColumnData previousMainAbiData = null;
List <ABIPCRColumnData> dataList = new List <ABIPCRColumnData>();
using (FileStream stream = File.Open(fileName, FileMode.Open, FileAccess.Read))
{
using (StreamReader reader = new StreamReader(stream))
{
while (!reader.EndOfStream)
{
string content = reader.ReadLine();
if (isRightFormat)
{
string[] values = content.Split(',');
if (values.Count() < 5)
{
break;
}
ABIPCRColumnData abiData = new ABIPCRColumnData();
abiData.Well = values[0];
abiData.Detector = values[2];
abiData.Ct = values[4];
abiData.XmlContent = "<Well>" + values[0] + "</Well><Detector>" + values[2] + "</Detector><Ct>" + values[4] + "</Ct>";
if (string.IsNullOrEmpty(abiData.Well))
{
if (previousMainAbiData.DataList == null)
{
previousMainAbiData.DataList = new List <PCRColumnData>();
}
previousMainAbiData.DataList.Add(abiData);
}
else
{
previousMainAbiData = abiData;
dataList.Add(previousMainAbiData);
}
}
if (!isRightFormat && content.StartsWith(formatTitle))
{
isRightFormat = true;
}
}
}
}
if (!isRightFormat)
{
return(isRightFormat);
}
Dictionary <int, DataRow> dc = controller.GetPCRPositionsByPlateID(plate.PlateID, currentExperimentId);
if (dc.Count != dataList.Count)
{
isRightPositionNumber = false;
if (!isRightPositionNumber)
{
MessageBoxResult selectResult = MessageBox.Show("PCR仪检测结果和混样数不一致，请检查操作是否正确？“是”将按现有规则导入", "系统提示", MessageBoxButton.YesNo);
if (selectResult == MessageBoxResult.No)
{
return(isRightFormat);
}
else
{
isRightPositionNumber = true;
}
}
}
foreach (ABIPCRColumnData abiData in dataList)
{
PCRTestResult info = new PCRTestResult();
info.ItemID = WanTaiObjectService.NewSequentialGuid();
info.RotationID = rotationId;
info.Position = controller.ChangeCharacterToPositionNumber(abiData.Well);
info.CreateTime = DateTime.Now;
//calculate
if (dc.ContainsKey(info.Position))
{
DataRow row = dc[info.Position];
bool isSingle = (int)row["TubeNumber"] == 1 ? true : false;
Tubetype tubeType = (Tubetype)((short)row["TubeType"]);
info.Result = checkPCRResult(abiData, row["TestName"].ToString(), isSingle, tubeType);
}
info.ExperimentID = currentExperimentId;
if (plate.BarCode != null)
{
info.BarCode = plate.BarCode;
}
info.PlateID = plate.PlateID;
string xmlContent = abiData.XmlContent;
if (abiData.DataList != null)
{
foreach (ABIPCRColumnData data in abiData.DataList)
{
xmlContent = xmlContent + data.XmlContent;
}
}
info.PCRContent = "<PCRContent>" + xmlContent + "</PCRContent>";
pcrResultList.Add(info);
}
return(isRightFormat);
}
