private void AddObjectsFromContours(Image <Bgr, Byte> image, List <Rectangle> contours, string wsi_path, int resolution_level)
{
// order bboxes from top to button / left to right (horizontal descending)
if (this.horizontal == true && this.descending == true)
{
// sort by x coordinate
contours.Sort((x, y) => x.Left.CompareTo(y.Left));
// than sort by y coordinate
contours.Sort((x, y) => x.Top.CompareTo(y.Top));
}
if (this.horizontal == true && this.descending == false)
{
contours.Sort((x, y) => x.Left.CompareTo(y.Left));
contours.Sort((x, y) => y.Top.CompareTo(x.Top));
}
if (this.horizontal == false && this.descending == true)
{
contours.Sort((x, y) => y.Left.CompareTo(x.Left));
contours.Sort((x, y) => x.Top.CompareTo(y.Top));
}
if (this.horizontal == true && this.descending == false)
{
contours.Sort((x, y) => y.Left.CompareTo(x.Left));
contours.Sort((x, y) => y.Top.CompareTo(x.Top));
}
for (int j = 0; j < contours.Count; j++)
{
Rectangle contour = contours[j];
WSIHistoObject obj = new WSIHistoObject();
using (Image <Bgr, Byte> subimage = image.Copy(new Rectangle(contour.Left, contour.Top, contour.Width, contour.Height)))
{
obj.PictureBox_SetImage(subimage.Bitmap, resolution_level);
}
int layerFactor = (int)(Math.Pow(2.0, (double)resolution_level));
obj.objRectangle = new Rectangle(contour.X * layerFactor, contour.Y * layerFactor, contour.Width * layerFactor, contour.Height * layerFactor);
obj.wsiImagePath = wsi_path;
obj.labelTitle.Text = System.IO.Path.GetFileNameWithoutExtension(wsi_path) + "_" + j.ToString();
obj.AllowDrop = true;
obj.DragDrop += new System.Windows.Forms.DragEventHandler(this.wsiHistoObject_DragDrop);
obj.DragOver += new System.Windows.Forms.DragEventHandler(this.wsiHistoObject_DragOver);
obj.MouseDown += new System.Windows.Forms.MouseEventHandler(this.wsiHistoObject_MouseDown);
obj.labelTitle.DragDrop += new System.Windows.Forms.DragEventHandler(this.wsiHistoObjectChild_DragDrop);
obj.labelTitle.DragOver += new System.Windows.Forms.DragEventHandler(this.wsiHistoObjectChild_DragOver);
obj.labelTitle.MouseDown += new System.Windows.Forms.MouseEventHandler(this.wsiHistoObjectChild_MouseDown);
obj.PictureBox_AddDragDropHandler(new System.Windows.Forms.DragEventHandler(this.wsiHistoObjectChild_DragDrop));
obj.PictureBox_AddDragOverHandler(new System.Windows.Forms.DragEventHandler(this.wsiHistoObjectChild_DragOver));
obj.PictureBox_AddMouseDownHandler(new System.Windows.Forms.MouseEventHandler(this.wsiHistoObjectChild_MouseDown));
obj.buttonRemove.MouseDown += new System.Windows.Forms.MouseEventHandler(this.wsiHistoObject_ButtonRem_Click);
objects.Add(obj);
this.panel1.Controls.Add(obj);
}
}
private void buttonCreateStack_Click(object sender, EventArgs e)
{
Directory.CreateDirectory(Path.Combine(ApplicationContext.OutputPath, textBox1.Text));
for (int i = 0; i < objects.Count; i++)
{
WSIHistoObject obj = objects[i];
SaveROIAsPNG(obj, (int)numericUpDown1.Value);
}
WriteStackV2((int)numericUpDown1.Value);
}
private void wsiHistoObject_ButtonRem_Click(object sender, MouseEventArgs e)
{
// remove object from form and objects list
WSIHistoObject obj = (WSIHistoObject)((Button)sender).Parent;
panel1.Controls.Remove(obj);
objects.Remove(obj);
EnableButtons();
// repaint form so the control disappears
this.Invalidate();
}
private string SaveROIAsPNG(WSIHistoObject histoObject, int resolution)
{
string path = null;
Cursor.Current = Cursors.WaitCursor;
try
{
using (VMscope.InteropCore.ImageStreaming.IStreamingImage image = VMscope.VirtualSlideAccess.Sdk.GetImage(histoObject.wsiImagePath))
{
int layerFactor = (int)(Math.Pow(2.0, resolution));
// is bitmap loaded correctly?
using (Bitmap bmp = image.GetImagePart(histoObject.objRectangle.X, histoObject.objRectangle.Y, histoObject.objRectangle.Width, histoObject.objRectangle.Height, (int)(histoObject.objRectangle.Width / layerFactor), (int)(histoObject.objRectangle.Height / layerFactor)))
{
/*using(BitmapViewer viewer = new BitmapViewer(bmp))
* {
* viewer.ShowDialog();
* }*/
// flip image so it fits the thumbnail
for (int k = 0; k < histoObject.NrOfRotations % 4; k++)
{
bmp.RotateFlip(RotateFlipType.Rotate90FlipNone);
}
if (histoObject.HorizontalFlip)
{
bmp.RotateFlip(RotateFlipType.RotateNoneFlipX);
}
// write image to file
path = Path.Combine(ApplicationContext.OutputPath, textBox1.Text, (histoObject.labelTitle.Text + ".png"));
bmp.Save(path, ImageFormat.Png);
Console.WriteLine("saved image " + histoObject.labelTitle.Text);
bmp.Dispose();
}
image.Dispose();
}
return(path);
} catch (Exception ex)
{
Console.WriteLine(ex);
MessageBox.Show(ex.Message);
return(path);
} finally
{
Cursor.Current = Cursors.Default;
}
}
private void wsiHistoObject_DragDrop(object sender, DragEventArgs e)
{
// get index of the target object
int index = objects.IndexOf((WSIHistoObject)sender);
if (index < 0)
{
index = objects.Count - 1;
}
WSIHistoObject data = (WSIHistoObject)e.Data.GetData(typeof(WSIHistoObject));
// move source object to target position
objects.Remove(data);
objects.Insert(index, data);
// repaint form so rearangement becomes visible
this.Invalidate();
}
private void buttonProceed_Click(object sender, EventArgs e)
{
List <string> toRegistrate = new List <string>();
Directory.CreateDirectory(Path.Combine(ApplicationContext.OutputPath, textBox1.Text));
for (int i = 0; i < objects.Count; i++)
{
WSIHistoObject obj = objects[i];
toRegistrate.Add(SaveROIAsPNG(obj, (int)numericUpDown1.Value));
}
WriteStackV2((int)numericUpDown1.Value);
this.Hide();
using (RegistrationForm form = new RegistrationForm(toRegistrate))
{
form.ShowDialog();
}
this.Close();
}