Beispiel #1
0
        private void aNOVAToolStripMenuItem1_Click(object sender, EventArgs e)
        {
            cGUI_ListClasses GUI_ListClasses = new cGUI_ListClasses();
            GUI_ListClasses.IsCheckBoxes = true;
            GUI_ListClasses.IsSelectAll = true;


            if (GUI_ListClasses.Run().IsSucceed == false) return;
            cExtendedTable ListClassSelected = GUI_ListClasses.GetOutPut();

            string SubTitle = "ANOVA (One-Way)";


            #region single plate and plate by plate

            cDesignerTab DT = new cDesignerTab();
            if ((ProcessModeCurrentPlateOnlyToolStripMenuItem.Checked) || (ProcessModeplateByPlateToolStripMenuItem.Checked)/*||(ProcessModeEntireScreeningToolStripMenuItem.Checked)*/)
            {
                List<cPlate> ListPlatesToProcess = new List<cPlate>();
                if ((ProcessModeplateByPlateToolStripMenuItem.Checked)/*||(ProcessModeEntireScreeningToolStripMenuItem.Checked)*/)
                {
                    foreach (cPlate TmpPlate in cGlobalInfo.CurrentScreening.ListPlatesActive)
                        ListPlatesToProcess.Add(TmpPlate);
                }
                else
                    ListPlatesToProcess.Add(cGlobalInfo.CurrentScreening.GetCurrentDisplayPlate());

                foreach (cPlate TmpPlate in ListPlatesToProcess)
                {
                    cExtendedTable NewTable1 = new cExtendedTable();
                    List<cListWells> ListListWells = new List<cListWells>();


                    int NumWells = 0;

                    for (int IdxClassWell = 0; IdxClassWell < ListClassSelected[0].Count; IdxClassWell++)
                    {
                        if (ListClassSelected[0][IdxClassWell] >= 1)
                        {
                            List<cWellClassType> LCT = new List<cWellClassType>();
                            LCT.Add(cGlobalInfo.ListWellClasses[IdxClassWell]);

                            cListWells TmpList = TmpPlate.ListWells.Filter(LCT);

                            if (TmpList.Count >= 3)
                            {
                                ListListWells.Add(TmpList);
                                NumWells += TmpList.Count;
                            }
                        }
                    }

                    if (ListListWells.Count <= 1)
                    {
                        if (ProcessModeCurrentPlateOnlyToolStripMenuItem.Checked)
                        {
                            MessageBox.Show("Insufficient number of control wells", "Error", MessageBoxButtons.OK, MessageBoxIcon.Error);
                            return;
                        }
                        else
                            continue;
                    }


                    cExtendedTable CompleteReport = null;
                    cExtendedList ListZ = new cExtendedList();
                    List<cDescriptorType> ListDescForZFactor = new List<cDescriptorType>();
                    List<string> ListNames = new List<string>();
                    int RealIdx = 0;
                    for (int IDxDesc = 0; IDxDesc < cGlobalInfo.CurrentScreening.ListDescriptors.Count; IDxDesc++)
                    {
                        if (!cGlobalInfo.CurrentScreening.ListDescriptors[IDxDesc].IsActive()) continue;

                        cExtendedTable TableForZ = new cExtendedTable();

                        foreach (var item in ListListWells)
                        {
                            List<cDescriptorType> LType = new List<cDescriptorType>();
                            LType.Add(cGlobalInfo.CurrentScreening.ListDescriptors[IDxDesc]);
                            cExtendedTable TTable = item.GetAverageDescriptorValues(LType, false, false);
                            TableForZ.Add(TTable[0]);
                        }
                        RealIdx++;

                        cANOVA ZF = new cANOVA();
                        ZF.SetInputData(TableForZ);
                        ZF.Run();
                        ListZ.Add(ZF.GetOutPut()[0][0]);

                        ZF.GetOutPut()[0].Name = cGlobalInfo.CurrentScreening.ListDescriptors[IDxDesc].GetName();

                        if (CompleteReport == null)
                            CompleteReport = new cExtendedTable(ZF.GetOutPut());
                        else
                        {
                            cMerge M = new cMerge();
                            M.IsHorizontal = true;
                            M.SetInputData(CompleteReport, ZF.GetOutPut());
                            M.Run();
                            CompleteReport = M.GetOutPut();
                        }

                        ListDescForZFactor.Add(cGlobalInfo.CurrentScreening.ListDescriptors[IDxDesc]);
                    }

                    cExtendedTable ET = new cExtendedTable(new cExtendedTable(ListZ));
                    ET[0].ListTags = new List<object>();
                    ET[0].ListTags.AddRange(ListDescForZFactor);
                    ET.Name = TmpPlate.GetName() + "\n" + SubTitle + " - " + NumWells + " wells";// +" - " +cGlobalInfo.ListWellClasses[IdxClassNeg].Name + " (" + NewTable1[0].Count + " wells) vs. " +cGlobalInfo.ListWellClasses[IdxClassPos].Name + " (" + NewTable2[0].Count + " wells)";
                    ET[0].Name = ET.Name;

                    cSort S = new cSort();
                    S.SetInputData(ET);
                    S.IsAscending = false;
                    S.ColumnIndexForSorting = 0;
                    S.Run();

                    //ZFactorList.Sort(delegate(cSimpleSignature p1, cSimpleSignature p2) { return p1.AverageValue.CompareTo(p2.AverageValue); });

                    cViewerGraph1D VG1 = new cViewerGraph1D();
                    VG1.SetInputData(S.GetOutPut());

                    VG1.Chart.LabelAxisY = SubTitle;
                    VG1.Chart.LabelAxisX = "Descriptor";
                    VG1.Chart.IsZoomableX = true;
                    VG1.Chart.IsBar = true;
                    VG1.Chart.IsBorder = true;
                    VG1.Chart.IsDisplayValues = true;
                    VG1.Chart.IsShadow = true;
                    VG1.Chart.MarkerSize = 4;

                    //VG1.Chart.Max
                    VG1.Title = TmpPlate.GetName();

                    // Classes.Base_Classes.General.cLineHorizontalForGraph VLZ05 = new Classes.Base_Classes.General.cLineHorizontalForGraph(.05);
                    // VLZ05.IsAllowMoving = true;
                    //  VG1.Chart.ListHorizontalLines.Add(VLZ05);

                    VG1.Run();

                    cDesignerSplitter DS = new cDesignerSplitter();
                    DS.Orientation = Orientation.Vertical;
                    DS.SetInputData(VG1.GetOutPut());


                    cTranspose T = new cTranspose();
                    T.SetInputData(CompleteReport);
                    T.Run();
                    CompleteReport = T.GetOutPut();
                    CompleteReport[0].Name = "P-Value";
                    CompleteReport[1].Name = "Is significant ?";

                    cSort S1 = new cSort();
                    S1.SetInputData(CompleteReport);
                    S1.ColumnIndexForSorting = 0;
                    S1.IsAscending = true;
                    S1.Run();



                    cViewerTable VT = new cViewerTable();
                    VT.SetInputData(S1.GetOutPut());
                    VT.DigitNumber = -1;
                    VT.Run();

                    DS.SetInputData(VT.GetOutPut());
                    DS.Title = TmpPlate.GetName();
                    DS.Run();

                    DT.SetInputData(DS.GetOutPut());
                }
                DT.Run();

                cDisplayToWindow CDW = new cDisplayToWindow();
                CDW.SetInputData(DT.GetOutPut());//VG1.GetOutPut());


                if (ProcessModeCurrentPlateOnlyToolStripMenuItem.Checked)
                    CDW.Title = SubTitle + " - " + ListPlatesToProcess[0].GetName();
                else
                    CDW.Title = SubTitle + " - " + ListPlatesToProcess.Count + " plates";

                CDW.Run();
                CDW.Display();
            }
            #endregion

            #region entire screening
            else if (ProcessModeEntireScreeningToolStripMenuItem.Checked)
            {
                List<cPlate> ListPlatesToProcess = new List<cPlate>();
                foreach (cPlate TmpPlate in cGlobalInfo.CurrentScreening.ListPlatesActive)
                    ListPlatesToProcess.Add(TmpPlate);

                cExtendedList ListZ = new cExtendedList();
                List<cPlate> ListPlatesForZFactor = new List<cPlate>();
                foreach (cPlate TmpPlate in ListPlatesToProcess)
                {
                    cListWells ListWellsToProcess1 = new cListWells(null);
                    cListWells ListWellsToProcess2 = new cListWells(null);

                    foreach (cWell item in TmpPlate.ListActiveWells)
                    {
                        if (item.GetCurrentClassIdx() != -1)
                        {
                            if (ListClassSelected[0][item.GetCurrentClassIdx()] == 1)
                                ListWellsToProcess1.Add(item);
                            if (ListClassSelected[1][item.GetCurrentClassIdx()] == 1)
                                ListWellsToProcess2.Add(item);
                        }
                    }

                    cExtendedTable NewTable1 = new cExtendedTable(ListWellsToProcess1, cGlobalInfo.CurrentScreening.ListDescriptors.GetDescriptorIndex(cGlobalInfo.CurrentScreening.ListDescriptors.GetActiveDescriptor()));
                    cExtendedTable NewTable2 = new cExtendedTable(ListWellsToProcess2, cGlobalInfo.CurrentScreening.ListDescriptors.GetDescriptorIndex(cGlobalInfo.CurrentScreening.ListDescriptors.GetActiveDescriptor()));
                    //cExtendedTable NewTable2 = new cExtendedTable(ListWellsToProcess2, false);

                    if ((NewTable1.Count == 0) || (NewTable1[0].Count < 2) || (NewTable2.Count == 0) || (NewTable2[0].Count < 2))
                    {
                        if (ProcessModeCurrentPlateOnlyToolStripMenuItem.Checked)
                        {
                            MessageBox.Show("Insufficient number of control wells", "Error", MessageBoxButtons.OK, MessageBoxIcon.Error);
                            return;
                        }
                        else
                            continue;
                    }

                    cExtendedTable TableForZ = new cExtendedTable();

                    TableForZ.Add(NewTable1[0]);
                    TableForZ.Add(NewTable2[0]);

                    cStudent_tTest ZF = new cStudent_tTest();
                    ZF.SetInputData(TableForZ);
                    ZF.Run();
                    double Zfactor = ZF.GetOutPut()[0][1];
                    ListZ.Add(Zfactor);

                    // update plate quality
                    //TmpPlate.ListProperties.UpdateValueByName("Quality", Math.Exp(Zfactor - 1));
                    //cProperty Prop = TmpPlate.ListProperties.FindByName("Quality");
                    //Prop.Info = ZF.GetInfo();

                    ListPlatesForZFactor.Add(TmpPlate);
                }


                cExtendedTable ET = new cExtendedTable(new cExtendedTable(ListZ));
                ET[0].ListTags = new List<object>();
                ET[0].ListTags.AddRange(ListPlatesForZFactor);
                ET.Name = SubTitle + " - " + cGlobalInfo.CurrentScreening.ListDescriptors[cGlobalInfo.CurrentScreening.ListDescriptors.CurrentSelectedDescriptorIdx].GetName();// +" - " +cGlobalInfo.ListWellClasses[IdxClassNeg].Name + /*" (" + NewTable1[0].Count + " wells)*/ " vs. " +cGlobalInfo.ListWellClasses[IdxClassPos].Name;// +" (" + NewTable2[0].Count + " wells)";
                ET[0].Name = ET.Name;

                cViewerGraph1D VG1 = new cViewerGraph1D();
                VG1.SetInputData(ET);

                VG1.Chart.LabelAxisY = SubTitle;
                VG1.Chart.LabelAxisX = "Plate";
                VG1.Chart.IsZoomableX = true;
                VG1.Chart.IsBar = true;
                VG1.Chart.IsBorder = true;
                VG1.Chart.IsDisplayValues = true;
                VG1.Chart.IsShadow = true;
                VG1.Chart.MarkerSize = 4;



                VG1.Title = cGlobalInfo.CurrentScreening.ListDescriptors[cGlobalInfo.CurrentScreening.ListDescriptors.CurrentSelectedDescriptorIdx].GetName();
                VG1.Run();

                cDisplayToWindow CDW = new cDisplayToWindow();
                CDW.SetInputData(VG1.GetOutPut());
                CDW.Title = SubTitle + " - " + ListPlatesToProcess.Count + " plates";
                CDW.Run();
                CDW.Display();
            #endregion
            }

        }
Beispiel #2
0
        private void PerformTestType(eTestType TestType)
        {

            #region extract classes of interest
            cGUI_ListClasses GUI_ListClasses = new cGUI_ListClasses();
            GUI_ListClasses.IsCheckBoxes = !_DescEvolCellByCellItem.Checked;
            GUI_ListClasses.IsSelectAll = true;

            if (GUI_ListClasses.Run().IsSucceed == false) return;
            cExtendedTable ListClassSelected = GUI_ListClasses.GetOutPut();

            if (ListClassSelected.Sum() < 1)
            {
                MessageBox.Show("At least one classe has to be selected.", "Error !", MessageBoxButtons.OK, MessageBoxIcon.Error);
                return;
            }
            #endregion

            cDesignerTab DT = new cDesignerTab();

            for (int i = 0; i < cGlobalInfo.CurrentScreening.ListDescriptors.Count; i++)
            {

                if (cGlobalInfo.CurrentScreening.ListDescriptors[i].IsActive() == false) continue;

                cDesignerSplitter DS = new cDesignerSplitter();
                DS.Orientation = Orientation.Vertical;
                cViewerTable VTable = new cViewerTable();

                cViewertext VT = new cViewertext();

                cExtendedTable FinalTable = new cExtendedTable(cGlobalInfo.ListWellClasses.Count, 0, 0);
                for (int j = 0; j < cGlobalInfo.ListWellClasses.Count; j++)
                {
                    FinalTable[j].Tag = cGlobalInfo.ListWellClasses[j];
                    FinalTable[j].Name = cGlobalInfo.ListWellClasses[j].Name;
                }

                cListWells ListWellsToProcess = new cListWells(null);
                cDescriptorType CurrentDesc = cGlobalInfo.CurrentScreening.ListDescriptors[i];
                // build the table
                foreach (cPlate CurrentPlate in cGlobalInfo.CurrentScreening.ListPlatesActive)
                {
                    foreach (cWell CurrentWell in CurrentPlate.ListActiveWells)
                    {
                        if ((CurrentWell.GetCurrentClassIdx() < 0) || (ListClassSelected[0][CurrentWell.GetCurrentClassIdx()] == 0)) continue;
                        {
                            FinalTable[CurrentWell.GetCurrentClassIdx()].Add(CurrentWell.GetAverageValue(CurrentDesc));
                            ListWellsToProcess.Add(CurrentWell);
                        }
                    }
                }

                cClean C = new cClean();
                C.SetInputData(FinalTable);
                C.Run();
                cExtendedTable NewTable = C.GetOutPut();

                cTwoSampleFTest TSFTest = null;
                cANOVA Anova = null;
                cTwoSampleUnpooledTTest TwoSampleTTest = null;
                cStudent_tTest StudentTTest = null;

                if (TestType == eTestType.F_TEST)
                {
                    TSFTest = new cTwoSampleFTest();
                    TSFTest.SetInputData(NewTable);
                    TSFTest.Run();
                    VTable.SetInputData(TSFTest.GetOutPut());
                    VT.SetInputData(TSFTest.GetInfo());
                }
                else if (TestType == eTestType.ANOVA)
                {
                    Anova = new cANOVA();
                    Anova.SetInputData(NewTable);
                    Anova.Run();
                    VTable.SetInputData(Anova.GetOutPut());
                    VT.SetInputData(Anova.GetInfo());
                }
                else if (TestType == eTestType.TWO_SAMPLES_T_TEST)
                {
                    TwoSampleTTest = new cTwoSampleUnpooledTTest();
                    TwoSampleTTest.SetInputData(NewTable);
                    TwoSampleTTest.Run();
                    VTable.SetInputData(TwoSampleTTest.GetOutPut());
                    VT.SetInputData(TwoSampleTTest.GetInfo());
                }
                else if (TestType == eTestType.STUDENT_T_TEST)
                {
                    StudentTTest = new cStudent_tTest();
                    StudentTTest.SetInputData(NewTable);
                    StudentTTest.Run();
                    VTable.SetInputData(StudentTTest.GetOutPut());
                    VT.SetInputData(StudentTTest.GetInfo());
                }
                VTable.DigitNumber = -1;
                VTable.Run();
                DS.SetInputData(VTable.GetOutPut());

                VT.Run();
                DS.SetInputData(VT.GetOutPut());
                DS.Run();
                DS.Title = cGlobalInfo.CurrentScreening.ListDescriptors[i].GetName();

                cDesignerSplitter DSMain = new cDesignerSplitter();
                DSMain.Orientation = Orientation.Horizontal;
                DSMain.SetInputData(DS.GetOutPut());

                // Compute and display associated Stacked histogram

                //CDW1.Title = CompleteScreening.ListDescriptors[CompleteScreening.ListDescriptors.CurrentSelectedDescriptorIdx].GetName() + " - Stacked Histogram - " + PlateList.Count + " plates";

                cExtendedTable TableForHisto = ListWellsToProcess.GetAverageDescriptorValues(i);
                TableForHisto.Name = "";

                cViewerStackedHistogram CV1 = new cViewerStackedHistogram();
                CV1.SetInputData(TableForHisto);
                CV1.Chart.LabelAxisX = cGlobalInfo.CurrentScreening.ListDescriptors[i].GetName();
                CV1.Chart.IsLegend = true;
                CV1.Run();

                DSMain.SetInputData(CV1.GetOutPut());
                DSMain.Title = cGlobalInfo.CurrentScreening.ListDescriptors[i].GetName();
                DSMain.Run();
                DT.SetInputData(DSMain.GetOutPut());
            }

            DT.Run();
            cDisplayToWindow DTW = new cDisplayToWindow();
            DTW.SetInputData(DT.GetOutPut());
            DTW.Title = "Classification Significance - " + TestType.ToString();
            DTW.Run();
            DTW.Display();

        }