public static void drift()
{
double Nf=1e4;
double MutationRatio=3e-10;
//int seed =1;
double tauKill=10;
double tauGrowNormal=20;
double tauGrowResistent=tauGrowNormal;
double N0mutation = 5e3;
double N0normal=5e3;
string FileName = "drift.txt";//Simulation"+ MutationRate.ToString("0.##E+0") +".txt";
System.IO.FileInfo myFile = new FileInfo(FileName);
myFile.Delete();
StreamWriter SR = new StreamWriter(FileName,false);
int sims=10;
Well[] myTest=new Well[sims] ;
Simulation[] mySimulation = new Simulation[sims];
SimulationParameters SP= new SimulationParameters( Nf,Nf,
MutationRatio,
tauKill,
tauKill ,
tauGrowNormal,
tauGrowResistent ,
0,200);
for (int sim=0;sim<sims;sim++)
{
myTest[sim] = new Well(N0normal,0,N0mutation);
mySimulation[sim]= new
Simulation(myTest[sim],
sim+90,
SP);
}
int rounds=200;
for(int round=0;round<rounds;round++)
{
double gen=round*Math.Log(100,2);
SR.Write("{0}\t",gen);
for (int sim=0;sim<sims;sim++)
{
myTest[sim]=mySimulation[sim].DoGrowing(myTest[sim]);
SR.Write("{0}\t",myTest[sim].NumberOfResistant);
myTest[sim]=mySimulation[sim].DoDilution(myTest[sim],100);
}
SR.Write("\n");
PrintPresentege(round,rounds);
}
SR.Close();
}
private static void DeferentDilutionCyclesSimulation(double MutationRatio,double Dilution,double N0Persisters)
{
double Nf=1e9;
int seed;
double tauNormalKill=10;
double tauPersisterKill=100;
double tauGrowNormal=20;
double tauGrowResistant=tauGrowNormal;
//double N0Persisters = 1e6;
double N0normal=Nf - N0Persisters;
double N0Resisters = 0;
double NormalPersistersFraction = N0Persisters/N0normal;
//string FileName = "CyclesSimulation"+ MutationRatio.ToString("0.##E+0") +".txt";
string FileName = "DeferentDilutionCycleOfFixation_" + MutationRatio.ToString("0.00E+000") +"_"+Dilution.ToString()+ "_" +N0Persisters.ToString("0.##E+000")+".txt";
System.IO.FileInfo myFile = new FileInfo(FileName);
myFile.Delete();
StreamWriter SR = new StreamWriter(FileName,false);
int Simulations = 10000;
for (int sim=0;sim < Simulations;sim++)
{
seed = sim;
SimulationParameters SP = new SimulationParameters(Nf,10*Nf,MutationRatio,tauNormalKill,tauPersisterKill ,tauGrowNormal,tauGrowResistant ,NormalPersistersFraction,Dilution);
Well NormalWell = new Well(N0normal,N0Persisters,N0Resisters);
Simulation NormalSimulation= new Simulation(NormalWell,seed,SP);
int cycle=0;
do
{
cycle++;
NormalWell = NormalSimulation.DoSycle();
}
while (NormalWell.NumberOfResistant<Nf);
SR.WriteLine(cycle);
}
SR.Close();
}
private static void DeferentDilutionCycleSimulation(double MutationRatio,double Dilution,double N0Persisters)
{
double Nf=1e9;
int seed=1;
double tauNormalKill=10;
double tauPersisterKill=100;
double tauGrowNormal=20;
double tauGrowResistant=tauGrowNormal;
//double N0Persisters = 1e8;
double N0normal=Nf - N0Persisters;
double N0Resisters = 0;
double NormalPersistersFraction = N0Persisters/N0normal;
string FileName = "OneExp_" + MutationRatio.ToString("0.##E+000") +"_"+Dilution.ToString() + "_" +N0Persisters.ToString("0.##E+000") +".txt";
System.IO.FileInfo myFile = new FileInfo(FileName);
myFile.Delete();
StreamWriter SR = new StreamWriter(FileName,false);
SimulationParameters SP = new SimulationParameters(Nf,10*Nf,MutationRatio,tauNormalKill,tauPersisterKill ,tauGrowNormal,tauGrowResistant ,NormalPersistersFraction,Dilution);
Well NormalWell = new Well(N0normal,N0Persisters,N0Resisters);
Simulation NormalSimulation= new Simulation(NormalWell,seed,SP);
int cycle=0;
do
{
cycle++;
NormalWell = NormalSimulation.DoSycle();
SR.WriteLine("{0}\t{1}\t{2}\t{3}",cycle,NormalWell.NumberOfNormal,NormalWell.NumberOfPersistent,NormalWell.NumberOfResistant);
}
while (NormalWell.NumberOfResistant<Nf);
SR.Close();
}
private static void CyclesSimulation()
{
double Nf=1e9;
double MutationRatio=1e-8 ;
int seed ;
double tauNormalKill=10;
double tauPersisterKill=100;
double tauGrowNormal=20;
double tauGrowResistant=tauGrowNormal;
double N0Persisters ;
double N0normal;
double N0Resisters;
double NormalPersistersFraction;
double HighPersistersFraction;
string FileName = "CyclesSimulation"+ MutationRatio.ToString("0.##E+0") +".txt";
System.IO.FileInfo myFile = new FileInfo(FileName);
myFile.Delete();
StreamWriter SR = new StreamWriter(FileName,false);
int Simulations = 10000;
for (int sim=0;sim < Simulations;sim++)
{
seed = sim;
N0Persisters = 1e6;
N0normal=Nf - N0Persisters;
N0Resisters = 0;
NormalPersistersFraction = N0Persisters/N0normal;
SimulationParameters SP ;
SP= new SimulationParameters(Nf,Nf,MutationRatio,tauNormalKill,tauPersisterKill ,tauGrowNormal,tauGrowResistant ,NormalPersistersFraction,200);
Well NormalWell = new Well(N0normal,N0Persisters,N0Resisters);
Simulation NormalSimulation= new Simulation(NormalWell,seed,SP);
do
{
NormalWell = NormalSimulation.DoSycle();
}
while (NormalWell.NumberOfResistant<Nf);
N0Persisters = 1e8;
N0normal=Nf - N0Persisters;
N0Resisters = 0;
HighPersistersFraction = N0Persisters/N0normal;
SP = new SimulationParameters(Nf,Nf,MutationRatio,tauNormalKill,tauPersisterKill ,tauGrowNormal,tauGrowResistant,HighPersistersFraction ,200);
Well HighPersistenceWell = new Well(N0normal,N0Persisters,N0Resisters);
Simulation HighPersistenceSimulation = new Simulation(HighPersistenceWell,seed,SP);
do
{
HighPersistenceWell = HighPersistenceSimulation.DoSycle();
}
while (HighPersistenceWell.NumberOfResistant<Nf);
SR.WriteLine("{0}\t{1}",NormalSimulation.Cycle,HighPersistenceSimulation.Cycle);
NormalSimulation = null;
HighPersistenceSimulation = null;
PrintPresentege(sim,Simulations);
}
SR.Close();
}
