private bool CheckSinglePlateSequential(PlateInfo plateInfo, ref string errMsg)
{
//var cellPositions = plateInfo.BarcodeDefinitions.Keys.ToList();
var cellPositions = plateInfo.BarcodeDefinitions.Where(x => x.Value != "").Select(x => x.Key).ToList();
var wellIDs = cellPositions.Select(x => x.WellID);
if (wellIDs.Count() == 0)
{
return(true);
}
HashSet <int> allIDs = new HashSet <int>(wellIDs);
int minWellID = wellIDs.Min();
int maxWellID = wellIDs.Max();
for (int id = minWellID; id < maxWellID; id++)
{
if (!allIDs.Contains(id))
{
errMsg = string.Format("位于{0}处的样品缺失！", new CellPosition(id - 1).AlphaInteger);
return(false);
}
}
return(true);
}
private void lstboxPlates_SelectionChanged(object sender, SelectionChangedEventArgs e)
{
SetHint("");
lstAssays.SelectedIndex = -1;
dataGridView.ClearSelection();
Utility.SaveDataGridView(dataGridView, curPlateInfo);
curPlateInfo = (PlateInfo)lstboxPlates.SelectedItem;
txtCheckDoc.DataContext = curPlateInfo;
Utility.InitDataGridView(dataGridView, curPlateInfo.BarcodeDefinitions.Count);
Utility.UpdateDataGridView(dataGridView, curPlateInfo);
int totalWell = curPlateInfo.MaxWellID;
bool bok = false;
string errMsg = "";
foreach (var pair in curPlateInfo.PredefinedBarcodes)
{
if (pair.Key.WellID <= totalWell)
{
var cell = dataGridView.Rows[pair.Key.rowIndex].Cells[pair.Key.colIndex];
bok = CheckConsist(pair.Key, cell, ref errMsg);
}
}
Trace.WriteLine(string.Format("Selection changed to plate: {0}", curPlateInfo.Name));
}
private void AddPlate()
{
SetHint("");
QueryName queryNameForm = new QueryName(plates.Select(x => x.Name).ToList());
var result = queryNameForm.ShowDialog();
if (!(bool)result)
{
return;
}
string newPlateName = queryNameForm.PlateName;
if (AlreadyExist(newPlateName))
{
SetHint(string.Format("板名为:{0}的微孔板已经存在！", newPlateName));
return;
}
PlateInfo newPlateInfo = new PlateInfo(newPlateName, queryNameForm.SelectedFormat, queryNameForm.PredefinedBarcodes);
Trace.WriteLine(string.Format("Create new plate：{0}, assay: {1}", newPlateName, queryNameForm.SelectedFormat));
plates.Add(newPlateInfo);
lstboxPlates.SelectedIndex = plates.Count - 1;
}
private void ParseSample(string line, PlateInfo plateInfo)
{
List <string> strs = line.Split('\t').ToList();
if (strs.All(x => x == ""))
{
return;
}
string well = strs[0];
string sampleName = strs[1];
CellPosition cellPosition = new CellPosition(well);
if (!plateInfo.IsWholePlate)//only 48 position
{
if (cellPosition.WellID > 48)
{
return;
}
}
string sAssay = strs[2];
if (sAssay != "")
{
assayName = sAssay;
}
plateInfo.BarcodeDefinitions[cellPosition] = sampleName;
}
static public void UpdateDataGridView(DataGridView dataGridView, PlateInfo plateInfo)
{
foreach (KeyValuePair <CellPosition, string> pair in plateInfo.BarcodeDefinitions)
{
CellPosition cellPos = pair.Key;
string barcode = pair.Value;
var cell = dataGridView.Rows[cellPos.rowIndex].Cells[cellPos.colIndex];
cell.Value = barcode;
}
}
public void MergeFrom(PlateInfo srcPlate, bool copy2FirstHalf)
{
for (int i = 0; i < 48; i++)
{
CellPosition srcCellPos = new CellPosition(i);
CellPosition dstCellPos = copy2FirstHalf ? srcCellPos : new CellPosition(i + 48);
if (srcPlate.BarcodeDefinitions.ContainsKey(srcCellPos))
{
barcodeDefinitions[dstCellPos] = srcPlate.BarcodeDefinitions[srcCellPos];
}
}
}
private void ParseGeneral(string generalInfo, out PlateInfo plateInfo)
{
List <string> strs = generalInfo.Split('\t').ToList();
string plateName = strs[0];
bool bMerged = false;
if (strs.Count == 8)
{
bMerged = strs[7].Contains(strings.merged);
}
plateInfo = new PlateInfo(plateName, null, null, bMerged);
plateInfo.PlateDescription = generalInfo;
}
private bool IsConsistentWithCheckDoc(PlateInfo plateInfo, ref string errMsg)
{
foreach (KeyValuePair <CellPosition, string> pair in plateInfo.BarcodeDefinitions)
{
if (plateInfo.PredefinedBarcodes.ContainsKey(pair.Key))
{
if (plateInfo.PredefinedBarcodes[pair.Key] != pair.Value)
{
errMsg += string.Format("板子:{0}中有{1}处与预定义不一致，请检查！", plateInfo.Name, pair.Key.AlphaInteger);
return(false);
}
}
}
return(true);
}
private bool SecondHalfContainsValue(PlateInfo plateInfo)
{
if (plateInfo.PlateDescription.Contains("merged"))
{
return(true);
}
foreach (var barcodeDef in plateInfo.BarcodeDefinitions)
{
if (barcodeDef.Key.WellID <= 48)
{
continue;
}
if (barcodeDef.Value != "")
{
return(true);
}
}
return(false);
}
private void SplitPlate()
{
SetHint("");
if (lstboxPlates.SelectedItem == null)
{
SetHint("请先选中一块板子！");
return;
}
Utility.SaveDataGridView(dataGridView, curPlateInfo);
var srcPlate = (PlateInfo)lstboxPlates.SelectedItem;
FormattedHeader formattedHeader = srcPlate.FileFormat;
PlateInfo firstHalf = new PlateInfo(srcPlate.Name + "_1", formattedHeader, null, false);
PlateInfo secondHalf = new PlateInfo(srcPlate.Name + "_2", formattedHeader, null, false);
firstHalf.SplitFrom(srcPlate, true);
secondHalf.SplitFrom(srcPlate, false);
plates.Add(firstHalf);
plates.Add(secondHalf);
SetHint("切分完成", false);
}
internal void SplitFrom(PlateInfo srcPlate, bool firstHalf)
{
int start = 0;
int end = 48;
if (!firstHalf)
{
start = 48;
end = 96;
}
for (int i = start; i < end; i++)
{
CellPosition srcCellPos = new CellPosition(i);
CellPosition dstCellPos = new CellPosition(i - start);
if (srcPlate.BarcodeDefinitions.ContainsKey(srcCellPos))
{
barcodeDefinitions[dstCellPos] = srcPlate.BarcodeDefinitions[srcCellPos];
}
}
}
private void AddHalf(bool firstHalf)
{
if (lstSrcPlates.SelectedItem == null)
{
SetHint("请选中一块板子！");
return;
}
PlateInfo curPlateInfo = (PlateInfo)lstSrcPlates.SelectedItem;
string sName = curPlateInfo.Name;
if (firstHalf)
{
firstPlate = curPlateInfo;
txtFirstHalf.Text = sName;
}
else
{
secondPlate = curPlateInfo;
txtSecondHalf.Text = sName;
}
}
private void btnOk_Click(object sender, RoutedEventArgs e)
{
if (firstPlate == null || secondPlate == null)
{
SetHint("请选中两块板子做Merge！");
return;
}
if (firstPlate.SampleDescription != secondPlate.SampleDescription)
{
SetHint("两块板的Assay类型不一样,您可以通过颜色区分。");
return;
}
bool containsValSecondHalf = SecondHalfContainsValue(firstPlate);
if (containsValSecondHalf)
{
SetHint("第一块板整版都设置过条码，不能Merge！");
return;
}
containsValSecondHalf = SecondHalfContainsValue(secondPlate);
if (containsValSecondHalf)
{
SetHint("第二块板整版都设置过条码，不能Merge！");
return;
}
FormattedHeader formattedHeader = new FormattedHeader(firstPlate.SampleDescription);
MergedPlate = new PlateInfo(firstPlate.Name + "_" + secondPlate.Name, formattedHeader, null, true);
MergedPlate.MergeFrom(firstPlate, true);
MergedPlate.MergeFrom(secondPlate, false);
this.DialogResult = true;
this.Close();
}
public void Write(string sFile, PlateInfo plateInfo)
{
List <string> allLines = new List <string>();
allLines.AddRange(fileHeader);
allLines.Add(plateInfo.PlateDescription);
allLines.Add("");
allLines.Add(sampleHeader);
plateInfo.BarcodeDefinitions = plateInfo.BarcodeDefinitions.OrderBy(x => Convert(x.Key)).ToDictionary(x => x.Key, x => x.Value);
string defaultText = "\t\t\t\t\t" + plateInfo.DefaultComment;
for (int i = 0; i < 96; i++)
{
CellPosition cellPos = new CellPosition(i);
if (plateInfo.BarcodeDefinitions.ContainsKey(cellPos))
{
string barcode = plateInfo.BarcodeDefinitions[cellPos];
string well = cellPos.AlphaInteger + "\t";
string sampleType = GetSampleType(barcode);
string line = well;
if (barcode != "")
{
line += barcode + "\t" + plateInfo.SampleDescription + "\t" + sampleType;
}
else
{
line = GetDefault(cellPos, defaultText);
}
allLines.Add(line);
}
else
{
allLines.Add(GetDefault(cellPos, defaultText));
}
}
File.WriteAllLines(sFile, allLines);
}
static public void SaveDataGridView(DataGridView dataGridView, PlateInfo curPlateInfo)
{
if (curPlateInfo == null)
{
return;
}
foreach (DataGridViewRow row in dataGridView.Rows)
{
foreach (DataGridViewCell cell in row.Cells)
{
CellPosition cellPos = new CellPosition(cell.ColumnIndex, cell.RowIndex);
if (!curPlateInfo.IsWholePlate)
{
if (cellPos.WellID > 48)
{
continue;
}
}
string sBarcode = cell.Value.ToString();
curPlateInfo.BarcodeDefinitions[cellPos] = sBarcode;
}
}
}
