private void DisplayMotif()
{
if (Motif == null)
{
return;
}
Settings settings = Settings.Load("default.settings");
int heightImage = 200;
int widthImage = 800;
if (settings != null)
{
heightImage = settings.MotifHeight;
widthImage = settings.MotifWidth;
}
if (Motif.PositiveColumns != null)
{
mdPositive.Image = Motif.GetPositiveMotif(widthImage, heightImage);
}
else if (Motif.Frequencies != null)
{
mdPositive.Image = Motif.GetFrequencyMotif(widthImage, heightImage);
}
else
{
mdPositive.Image = null;
}
if (Motif.NegativeColumns != null)
{
mdNegative.Image = Motif.GetNegativeMotif(widthImage, heightImage);
}
else
{
mdNegative.Visible = false;
mdNegative.Image = null;
}
if (Motif.PositiveColumns != null)
{
mdChart.Image = Motif.GetBarChart(pMotif.Width - 6);
}
else
{
mdChart.Visible = false;
}
ePositiveThreshold.Text = Motif.PositiveThreshold.ToString();
toolTip1.SetToolTip(eScorerPosThreshold, string.Format("Range: [{0:N2}, {1:N2}]", Motif.PositiveThreshold, Motif.MaxPosWeight));
eNegativeThreshold.Text = Motif.NegativeThreshold.ToString();
toolTip1.SetToolTip(eScorerNegThreshold, string.Format("Range: [{0:N2}, {1:N2}]", 0, Motif.NegativeThreshold));
eWildtype.Text = Motif.WildTypePeptide;
eTargetPosition.Text = "";
eTargetAminoAcid.Text = "";
}
private void DrawMotifs()
{
ClearMotifs();
Settings settings = Settings.Load("default.settings");
int heightImage = 200;
int widthImage = 800;
if (settings != null)
{
heightImage = settings.MotifHeight;
widthImage = settings.MotifWidth;
}
int midpoint = peptidelength / 2;
if (Int32.TryParse(eKeyPosition.Text, out keyPos) && keyPos <= peptidelength && keyPos > 0)
{
midpoint = keyPos - 1;
}
else
{
keyPos = midpoint + 1;
eKeyPosition.Text = keyPos.ToString();
}
if (!Char.TryParse(eAminoAcid.Text.Trim(), out keyAA))
{
keyAA = ' ';
eAminoAcid.Text = keyAA.ToString();
}
else if (Char.IsLower(keyAA))
{
keyAA = Char.ToUpper(keyAA);
}
if (keyAA != ' ')
{
List <string> mainList = Peptides.Where(s => s[keyPos - 1] == keyAA).ToList();
List <string> shiftedList = Analyzer.ShiftPeptides(Peptides.Where(s => s[keyPos - 1] != keyAA).ToList(), keyAA, peptidelength, keyPos - 1, out List <string> replacements);
MainMotif = new Motif(mainList, peptidelength);
MainMotif.FreqThreshold = threshold;
Bitmap bm = MainMotif.GetFrequencyMotif(widthImage, heightImage);
mdMain.Image = bm;
ShiftedMotif = new Motif(shiftedList, peptidelength);
ShiftedMotif.FreqThreshold = threshold;
bm = ShiftedMotif.GetFrequencyMotif(widthImage, heightImage);
mdShifted.Image = bm;
mdShifted.Visible = true;
eOutput.Text += String.Join("\r\nInfo: ", replacements) + "\r\n";
eOutput.Text += "Motifs are created succesfully.\r\n";
}
else
{
MainMotif = new Motif(Peptides, peptidelength);
MainMotif.FreqThreshold = threshold;
Bitmap bm = MainMotif.GetFrequencyMotif(widthImage, heightImage);
mdMain.Image = bm;
mdShifted.Visible = false;
eOutput.Text += "Motif is created succesfully.\r\n";
}
}
private bool DrawMotifs()
{
try
{
ClearMotifs();
if (PA == null)
{
return(true);
}
Settings settings = Settings.Load("default.settings");
int heightImage = 200;
int widthImage = 800;
if (settings != null)
{
heightImage = settings.MotifHeight;
widthImage = settings.MotifWidth;
}
int midpoint = peptidelength / 2;
if (Int32.TryParse(eKeyPosition.Text, out int keyPos) && keyPos <= peptidelength && keyPos > 0)
{
midpoint = keyPos - 1;
}
else
{
keyPos = midpoint + 1;
eKeyPosition.Text = keyPos.ToString();
}
PA.KeyPosition = keyPos;
if (!Char.TryParse(eAminoAcid.Text.Trim(), out char keyAA))
{
keyAA = ' ';
eAminoAcid.Text = keyAA.ToString();
}
else if (Char.IsLower(keyAA))
{
keyAA = Char.ToUpper(keyAA);
}
PA.KeyAA = keyAA;
if (keyAA != ' ')
{
List <string> mainList = PA.ModifiedPeptides.Where(s => s[keyPos - 1] == keyAA).ToList();
List <string> shiftedList = Analyzer.ShiftPeptides(PA.ModifiedPeptides.Where(s => s[keyPos - 1] != keyAA).ToList(), keyAA, peptidelength, keyPos - 1, out List <string> replacements);
MotifMain = new Motif(mainList, peptidelength);
MotifMain.FreqThreshold = PA.FrequencyThreshold;
Bitmap bm = MotifMain.GetFrequencyMotif(widthImage, heightImage);
mdMain.Image = bm;
MotifShifted = new Motif(shiftedList, peptidelength);
MotifShifted.FreqThreshold = PA.FrequencyThreshold;
bm = MotifShifted.GetFrequencyMotif(widthImage, heightImage);
mdShifted.Image = bm;
mdShifted.Visible = true;
}
else
{
MotifMain = new Motif(PA.ModifiedPeptides, peptidelength);
MotifMain.FreqThreshold = PA.FrequencyThreshold;
Bitmap bm = MotifMain.GetFrequencyMotif(widthImage, heightImage);
mdMain.Image = bm;
mdShifted.Visible = false;
}
return(true);
}
catch { return(false); }
}