/// <summary>
/// This method is called when the user wants to run BLAST on a sequence
/// </summary>
private void OnBlastSequence()
{
// Get the full sequence, or the selected fragment
ISequence sequence = !string.IsNullOrEmpty(SelectedSequenceFragment)
? new Sequence(SelectedSequence.RawSequence.Alphabet, SelectedSequenceFragment)
: SelectedSequence.RawSequence;
IBlastServiceHandler blastServiceHandler = SelectedBlastAlgorithm;
BlastParameters bp = new BlastParameters();
bp.Add("Program", "blastn");
bp.Add("Database", "nr");
bp.Add("Expect", "10.0");
blastServiceHandler.RequestCompleted += new EventHandler <BlastRequestCompletedEventArgs>(BlastServiceHandlerRequestCompleted);
try
{
IsRunning = blastServiceHandler.SubmitRequest(sequence, bp);
if (string.IsNullOrEmpty(IsRunning))
{
ShowError("Failed to execute Blast", "No results returned", null);
}
}
catch (Exception ex)
{
ShowError("Failed to execute Blast", "Error occurred", ex);
ResultHits = null;
}
}
/// <summary>
/// This method is called when the user wants to run BLAST on a sequence
/// </summary>
private void OnBlastSequence()
{
// Get the full sequence, or the selected fragment
ISequence sequence = !string.IsNullOrEmpty(SelectedSequenceFragment)
? new Sequence(SelectedSequence.RawSequence.Alphabet, SelectedSequenceFragment)
: SelectedSequence.RawSequence;
IBlastServiceHandler blastServiceHandler = SelectedBlastAlgorithm;
BlastParameters bp = new BlastParameters();
bp.Add("Program", "blastn");
bp.Add("Database", "nr");
bp.Add("Expect", "10.0");
try
{
string resultKey = blastServiceHandler.SubmitRequest(sequence, bp);
if (string.IsNullOrEmpty(resultKey))
{
ShowError("Failed to execute Blast", "No results returned", null);
}
else
{
IList <BlastResult> results =
blastServiceHandler.FetchResultsSync(resultKey, bp);
if (results == null || results.Count == 0)
{
MessageBox.Show("No Results returned.");
ResultHits = null;
}
else
{
ResultHits = results[0].Records[0].Hits;
}
}
}
catch (Exception ex)
{
ShowError("Failed to execute Blast", "Error occurred", ex);
ResultHits = null;
}
}
/// <summary>
/// This method is called when the user wants to run BLAST on a sequence
/// </summary>
private void OnBlastSequence()
{
// Get the full sequence, or the selected fragment
ISequence sequence = !string.IsNullOrEmpty(SelectedSequenceFragment)
? new Sequence(SelectedSequence.RawSequence.Alphabet, SelectedSequenceFragment)
: SelectedSequence.RawSequence;
IBlastServiceHandler blastServiceHandler = SelectedBlastAlgorithm;
BlastParameters bp = new BlastParameters();
foreach (var item in BlastParameters.Where(kv => !string.IsNullOrEmpty(kv.Value)))
{
bp.Add(item.Key, item.Value);
}
if (!string.IsNullOrEmpty(Url))
{
blastServiceHandler.Configuration.Connection = new Uri(Url);
if (Url.StartsWith("https"))
{
blastServiceHandler.Configuration.UseHttps = true;
}
}
blastServiceHandler.Configuration.UseBrowserProxy = UseBrowserProxy;
blastServiceHandler.RequestCompleted += new EventHandler <BlastRequestCompletedEventArgs>(BlastServiceHandlerRequestCompleted);
try
{
IsRunning = blastServiceHandler.SubmitRequest(sequence, bp);
if (string.IsNullOrEmpty(IsRunning))
{
ShowError("Failed to execute Blast", "No results returned", null);
}
}
catch (Exception ex)
{
ShowError("Failed to execute Blast", "Error occurred", ex);
ResultHits = null;
}
}
