Пример #1
0
        public void GetTotalExonLength_MultipleExons()
        {
            const int expectedLength = 300;
            int       observedLength = ExonUtilities.GetTotalExonLength(_transcriptRegions);

            Assert.Equal(expectedLength, observedLength);
        }
Пример #2
0
        public static ITranscript Read(BufferedBinaryReader reader,
                                       IDictionary <ushort, IChromosome> chromosomeIndexDictionary, IGene[] cacheGenes,
                                       ITranscriptRegion[] cacheTranscriptRegions, IInterval[] cacheMirnas, string[] cachePeptideSeqs)
        {
            // transcript
            var referenceIndex = reader.ReadOptUInt16();
            var start          = reader.ReadOptInt32();
            var end            = reader.ReadOptInt32();
            var id             = CompactId.Read(reader);

            // gene
            var geneIndex = reader.ReadOptInt32();
            var gene      = cacheGenes[geneIndex];

            // encoded data
            var encoded = EncodedTranscriptData.Read(reader);

            // transcript regions
            var    transcriptRegions = encoded.HasTranscriptRegions ? ReadIndices(reader, cacheTranscriptRegions) : null;
            ushort numExons          = reader.ReadOptUInt16();

            // protein function predictions
            int siftIndex     = encoded.HasSift     ? reader.ReadOptInt32() : -1;
            int polyphenIndex = encoded.HasPolyPhen ? reader.ReadOptInt32() : -1;

            // translation
            var translation = encoded.HasTranslation ? DataStructures.Translation.Read(reader, cachePeptideSeqs) : null;

            // attributes
            var mirnas          = encoded.HasMirnas          ? ReadIndices(reader, cacheMirnas)         : null;
            var rnaEdits        = encoded.HasRnaEdits        ? ReadItems(reader, RnaEdit.Read)          : null;
            var selenocysteines = encoded.HasSelenocysteines ? ReadItems(reader, x => x.ReadOptInt32()) : null;

            return(new Transcript(chromosomeIndexDictionary[referenceIndex], start, end, id, translation,
                                  encoded.BioType, gene, ExonUtilities.GetTotalExonLength(transcriptRegions), encoded.StartExonPhase,
                                  encoded.IsCanonical, transcriptRegions, numExons, mirnas, siftIndex, polyphenIndex,
                                  encoded.TranscriptSource, encoded.CdsStartNotFound, encoded.CdsEndNotFound, selenocysteines, rnaEdits));
        }