public KRASStandardReflexKRASWithBRAFResult(string reportNo, YellowstonePathology.Business.Test.AccessionOrder accessionOrder)
: base(reportNo, accessionOrder)
{
this.m_Method = "DNA was isolated from cells or microdissection-enriched FFPE tissue. Tumor in FFPE must be present in at least 20% of the tissue. " +
"Mutations were evaluated for entire KRAS exons 2 and 3 high-sensitivity Sanger sequencing which improves the lower detection limit in mutation hotspot " +
"regions to approximately 1% abnormal DNA. This includes codons 12, 13, 14, and 61. Mutation detection outside these hotspot regions has a typical lower " +
"detection limit of 10-15% mutated KRAS in a wild-type background. The patient’s sequence is compared to the GenBank database: AF493917. The DNA was also " +
"evaluated for the presence of BRAF V600E/K by PCR and fragment size anaylsis. Various factors including quantity and quality of nucleic acid, sample " +
"preparation and sample age can affect assay performance.";
YellowstonePathology.Business.Test.BRAFV600EK.BRAFV600EKTest brafV600EKTest = new BRAFV600EK.BRAFV600EKTest();
this.m_BRAFV600EKTestOrder = (YellowstonePathology.Business.Test.BRAFV600EK.BRAFV600EKTestOrder)accessionOrder.PanelSetOrderCollection.GetPanelSetOrder(brafV600EKTest.PanelSetId, this.m_KRASStandardReflexTestOrder.OrderedOnId, true);
if (this.KRASStandardTestOrder.Final == true)
{
this.m_KRASStandardResult = this.m_KRASStandardTestOrder.Result;
}
else
{
this.m_KRASStandardResult = KRASStandardReflexResult.PendingResult;
}
if (this.m_BRAFV600EKTestOrder.Final == true)
{
this.m_BRAFV600EKResult = this.m_BRAFV600EKTestOrder.Result;
}
else
{
this.m_BRAFV600EKResult = KRASStandardReflexResult.PendingResult;
}
}
public KRASStandardReflexKRASWithBRAFResult(string reportNo, YellowstonePathology.Business.Test.AccessionOrder accessionOrder) : base(reportNo, accessionOrder)
{
this.m_Method = "DNA was isolated from cells or microdissection-enriched FFPE tissue. Tumor in FFPE must be present in at least 20% of the tissue. " +
"Mutations were evaluated for entire KRAS exons 2 and 3 high-sensitivity Sanger sequencing which improves the lower detection limit in mutation hotspot " +
"regions to approximately 1% abnormal DNA. This includes codons 12, 13, 14, and 61. Mutation detection outside these hotspot regions has a typical lower " +
"detection limit of 10-15% mutated KRAS in a wild-type background. The patient’s sequence is compared to the GenBank database: AF493917. The DNA was also " +
"evaluated for the presence of BRAF V600E/K by PCR and fragment size anaylsis. Various factors including quantity and quality of nucleic acid, sample " +
"preparation and sample age can affect assay performance.";
YellowstonePathology.Business.Test.BRAFV600EK.BRAFV600EKTest brafV600EKTest = new BRAFV600EK.BRAFV600EKTest();
this.m_BRAFV600EKTestOrder = (YellowstonePathology.Business.Test.BRAFV600EK.BRAFV600EKTestOrder)accessionOrder.PanelSetOrderCollection.GetPanelSetOrder(brafV600EKTest.PanelSetId, this.m_KRASStandardReflexTestOrder.OrderedOnId, true);
if (this.KRASStandardTestOrder.Final == true)
{
this.m_KRASStandardResult = this.m_KRASStandardTestOrder.Result;
}
else
{
this.m_KRASStandardResult = KRASStandardReflexResult.PendingResult;
}
if (this.m_BRAFV600EKTestOrder.Final == true)
{
this.m_BRAFV600EKResult = this.m_BRAFV600EKTestOrder.Result;
}
else
{
this.m_BRAFV600EKResult = KRASStandardReflexResult.PendingResult;
}
}
public void UpdateFromChildren(YellowstonePathology.Business.Test.AccessionOrder accessionOrder, YellowstonePathology.Business.Test.KRASStandard.KRASStandardTestOrder testOrder)
{
this.Indication = testOrder.Indication;
this.IndicationComment = testOrder.IndicationComment;
this.TumorNucleiPercentage = testOrder.TumorNucleiPercentage;
this.Comment = testOrder.Comment;
YellowstonePathology.Business.Test.BRAFV600EK.BRAFV600EKTest brafV600EKTest = new BRAFV600EK.BRAFV600EKTest();
if (accessionOrder.PanelSetOrderCollection.Exists(brafV600EKTest.PanelSetId) == true)
{
YellowstonePathology.Business.Test.BRAFV600EK.BRAFV600EKTestOrder brafV600EKTestOrder = (YellowstonePathology.Business.Test.BRAFV600EK.BRAFV600EKTestOrder)accessionOrder.PanelSetOrderCollection.GetPanelSetOrder(brafV600EKTest.PanelSetId);
brafV600EKTestOrder.Indication = testOrder.Indication;
brafV600EKTestOrder.IndicationComment = testOrder.IndicationComment;
brafV600EKTestOrder.TumorNucleiPercentage = testOrder.TumorNucleiPercentage;
brafV600EKTestOrder.Comment = testOrder.Comment;
}
}
public static KRASStandardReflexResult GetResult(string reportNo, YellowstonePathology.Business.Test.AccessionOrder accessionOrder)
{
KRASStandardReflexResult result = null;
YellowstonePathology.Business.Test.KRASStandardReflex.KRASStandardReflexTestOrder krasStandardReflexTestOrder = (YellowstonePathology.Business.Test.KRASStandardReflex.KRASStandardReflexTestOrder)accessionOrder.PanelSetOrderCollection.GetPanelSetOrder(reportNo);
YellowstonePathology.Business.Test.KRASStandard.KRASStandardTest krasStandardTest = new KRASStandard.KRASStandardTest();
YellowstonePathology.Business.Test.KRASStandard.KRASStandardTestOrder krasStandardTestOrder = (YellowstonePathology.Business.Test.KRASStandard.KRASStandardTestOrder)accessionOrder.PanelSetOrderCollection.GetPanelSetOrder(krasStandardTest.PanelSetId, krasStandardReflexTestOrder.OrderedOnId, true);
YellowstonePathology.Business.Test.BRAFV600EK.BRAFV600EKTest brafV600EKTest = new BRAFV600EK.BRAFV600EKTest();
if (accessionOrder.PanelSetOrderCollection.Exists(brafV600EKTest.PanelSetId, krasStandardReflexTestOrder.OrderedOnId, true) == false)
{
result = new KRASStandardReflexKRASOnlyResult(reportNo, accessionOrder);
}
else
{
YellowstonePathology.Business.Test.BRAFV600EK.BRAFV600EKTestOrder brafV600EKTestOrder = (YellowstonePathology.Business.Test.BRAFV600EK.BRAFV600EKTestOrder)accessionOrder.PanelSetOrderCollection.GetPanelSetOrder(brafV600EKTest.PanelSetId, krasStandardReflexTestOrder.OrderedOnId, true);
YellowstonePathology.Business.Test.BRAFV600EK.BRAFV600EKNotDetectedResult brafV600EKNotDetectedResult = new BRAFV600EK.BRAFV600EKNotDetectedResult();
YellowstonePathology.Business.Test.KRASStandard.KRASStandardNotDetectedResult krasStandardNotDetectedResult = new KRASStandard.KRASStandardNotDetectedResult();
YellowstonePathology.Business.Test.BRAFV600EK.BRAFV600EKDetectedResult brafV600EKDetectedResult = new BRAFV600EK.BRAFV600EKDetectedResult();
if (krasStandardTestOrder.ResultCode == krasStandardNotDetectedResult.ResultCode &&
brafV600EKTestOrder.ResultCode == brafV600EKNotDetectedResult.ResultCode)
{
result = new KRASStandardReflexBothNotDetectedResult(reportNo, accessionOrder);
}
else if (krasStandardTestOrder.ResultCode == krasStandardNotDetectedResult.ResultCode &&
brafV600EKTestOrder.ResultCode == brafV600EKDetectedResult.ResultCode)
{
result = new KRASStandardReflexKRASNotDetecedBRAFDetectedResult(reportNo, accessionOrder);
}
else
{
result = new KRASStandardReflexKRASWithBRAFResult(reportNo, accessionOrder);
}
}
return(result);
}
public static KRASStandardReflexResult GetResult(string reportNo, YellowstonePathology.Business.Test.AccessionOrder accessionOrder)
{
KRASStandardReflexResult result = null;
YellowstonePathology.Business.Test.KRASStandardReflex.KRASStandardReflexTestOrder krasStandardReflexTestOrder = (YellowstonePathology.Business.Test.KRASStandardReflex.KRASStandardReflexTestOrder)accessionOrder.PanelSetOrderCollection.GetPanelSetOrder(reportNo);
YellowstonePathology.Business.Test.KRASStandard.KRASStandardTest krasStandardTest = new KRASStandard.KRASStandardTest();
YellowstonePathology.Business.Test.KRASStandard.KRASStandardTestOrder krasStandardTestOrder = (YellowstonePathology.Business.Test.KRASStandard.KRASStandardTestOrder)accessionOrder.PanelSetOrderCollection.GetPanelSetOrder(krasStandardTest.PanelSetId, krasStandardReflexTestOrder.OrderedOnId, true);
YellowstonePathology.Business.Test.BRAFV600EK.BRAFV600EKTest brafV600EKTest = new BRAFV600EK.BRAFV600EKTest();
if (accessionOrder.PanelSetOrderCollection.Exists(brafV600EKTest.PanelSetId, krasStandardReflexTestOrder.OrderedOnId, true) == false)
{
result = new KRASStandardReflexKRASOnlyResult(reportNo, accessionOrder);
}
else
{
YellowstonePathology.Business.Test.BRAFV600EK.BRAFV600EKTestOrder brafV600EKTestOrder = (YellowstonePathology.Business.Test.BRAFV600EK.BRAFV600EKTestOrder)accessionOrder.PanelSetOrderCollection.GetPanelSetOrder(brafV600EKTest.PanelSetId, krasStandardReflexTestOrder.OrderedOnId, true);
YellowstonePathology.Business.Test.BRAFV600EK.BRAFV600EKNotDetectedResult brafV600EKNotDetectedResult = new BRAFV600EK.BRAFV600EKNotDetectedResult();
YellowstonePathology.Business.Test.KRASStandard.KRASStandardNotDetectedResult krasStandardNotDetectedResult = new KRASStandard.KRASStandardNotDetectedResult();
YellowstonePathology.Business.Test.BRAFV600EK.BRAFV600EKDetectedResult brafV600EKDetectedResult = new BRAFV600EK.BRAFV600EKDetectedResult();
if (krasStandardTestOrder.ResultCode == krasStandardNotDetectedResult.ResultCode &&
brafV600EKTestOrder.ResultCode == brafV600EKNotDetectedResult.ResultCode)
{
result = new KRASStandardReflexBothNotDetectedResult(reportNo, accessionOrder);
}
else if (krasStandardTestOrder.ResultCode == krasStandardNotDetectedResult.ResultCode &&
brafV600EKTestOrder.ResultCode == brafV600EKDetectedResult.ResultCode)
{
result = new KRASStandardReflexKRASNotDetecedBRAFDetectedResult(reportNo, accessionOrder);
}
else
{
result = new KRASStandardReflexKRASWithBRAFResult(reportNo, accessionOrder);
}
}
return result;
}
public void UpdateFromChildren(YellowstonePathology.Business.Test.AccessionOrder accessionOrder, YellowstonePathology.Business.Test.KRASStandard.KRASStandardTestOrder testOrder)
{
this.Indication = testOrder.Indication;
this.IndicationComment = testOrder.IndicationComment;
this.TumorNucleiPercentage = testOrder.TumorNucleiPercentage;
this.Comment = testOrder.Comment;
YellowstonePathology.Business.Test.BRAFV600EK.BRAFV600EKTest brafV600EKTest = new BRAFV600EK.BRAFV600EKTest();
if (accessionOrder.PanelSetOrderCollection.Exists(brafV600EKTest.PanelSetId) == true)
{
YellowstonePathology.Business.Test.BRAFV600EK.BRAFV600EKTestOrder brafV600EKTestOrder = (YellowstonePathology.Business.Test.BRAFV600EK.BRAFV600EKTestOrder)accessionOrder.PanelSetOrderCollection.GetPanelSetOrder(brafV600EKTest.PanelSetId);
brafV600EKTestOrder.Indication = testOrder.Indication;
brafV600EKTestOrder.IndicationComment = testOrder.IndicationComment;
brafV600EKTestOrder.TumorNucleiPercentage = testOrder.TumorNucleiPercentage;
brafV600EKTestOrder.Comment = testOrder.Comment;
}
}
