public frmBatchGenImg(List <string> argResultFiles, string argExportRootPath, enumGlycanLabelingMethod argLabelingMethod, bool argIndividualImg, bool argQuantImg)
{
InitializeComponent();
ResultFiles = argResultFiles;
isIndividualImg = argIndividualImg;
isQuantImg = argQuantImg;
CompletedCount = 0;
ExportFilePath = argExportRootPath;
LabelingMethod = argLabelingMethod;
bgWorkerGenerateImages.RunWorkerAsync();
}
public frmBatchGenImg(List<string> argResultFiles, string argExportRootPath, enumGlycanLabelingMethod argLabelingMethod, bool argIndividualImg, bool argQuantImg)
{
InitializeComponent();
ResultFiles = argResultFiles;
isIndividualImg = argIndividualImg;
isQuantImg = argQuantImg;
CompletedCount = 0;
ExportFilePath = argExportRootPath;
LabelingMethod = argLabelingMethod;
bgWorkerGenerateImages.RunWorkerAsync();
}
public static void GenQuantImg(string argQuantFile, enumGlycanLabelingMethod argLabelingMethod,
string argExportFolder)
{
string Dir = argExportFolder + "\\Pic";
if (!Directory.Exists(Dir))
{
Directory.CreateDirectory(Dir);
}
List <Color> LstColor = new List <Color>()
{
Color.DarkCyan,
Color.DarkGoldenrod,
Color.DarkGray,
Color.DarkGreen,
Color.DarkKhaki,
Color.DarkMagenta,
Color.DarkOliveGreen,
Color.DarkOrchid,
Color.DarkRed,
Color.DarkSalmon,
Color.DarkSeaGreen,
Color.DarkSlateBlue,
Color.DarkSlateGray,
Color.DarkTurquoise,
Color.DarkViolet,
Color.DeepPink,
Color.DeepSkyBlue
};
//Get Title
Dictionary <string, int> dictTitle = new Dictionary <string, int>();
StreamReader sr = new StreamReader(argQuantFile);
string tmp = "";
//bool isLabeling = false;
tmp = sr.ReadLine();
#region Read Title
string[] tmpAry = tmp.Split(',');
//string LabelTitle = "";
dictTitle.Add("Glycan", 0);
List <string> lstLabelingTag = new List <string>();
if (argLabelingMethod == enumGlycanLabelingMethod.DRAG)
{
lstLabelingTag.Add("DRAG_Light(Adjusted 1)");
lstLabelingTag.Add("DRAG_Heavy(Adjusted 1)");
}
else if (argLabelingMethod == enumGlycanLabelingMethod.MultiplexPermethylated)
{
lstLabelingTag.Add("MP_CH3");
lstLabelingTag.Add("MP_CH2D");
lstLabelingTag.Add("MP_CHD2");
lstLabelingTag.Add("MP_CD3");
lstLabelingTag.Add("MP_13CH3");
lstLabelingTag.Add("MP_13CH2D");
lstLabelingTag.Add("MP_13CHD2");
lstLabelingTag.Add("MP_13CD3");
}
for (int i = 1; i < tmpAry.Length; i++)
{
if (argLabelingMethod == enumGlycanLabelingMethod.MultiplexPermethylated &&
tmpAry[i].StartsWith("MP"))
{
dictTitle.Add(tmpAry[i], i + 3);
// MP_CH3 Normalization Factor Estimated Purity Normalizted and Adjusted Abundance (Adjusted Factor=1)
}
else if (argLabelingMethod == enumGlycanLabelingMethod.DRAG &&
tmpAry[i].Contains("DRAG_Light(Adjusted 1)") ||
tmpAry[i].Contains("DRAG_Heavy(Adjusted 1)"))
{
dictTitle.Add(tmpAry[i], i);
}
}
#endregion
#region Get Data
Dictionary <string, Dictionary <string, double> > dictData =
new Dictionary <string, Dictionary <string, double> >();
while (!sr.EndOfStream)
{
tmp = sr.ReadLine();
tmpAry = tmp.Split(',');
string GlycanKey = tmpAry[dictTitle["Glycan"]];
if (!dictData.ContainsKey(GlycanKey))
{
dictData.Add(GlycanKey, new Dictionary <string, double>());
}
foreach (string LabelTag in lstLabelingTag)
{
if (!dictTitle.ContainsKey(LabelTag))
{
continue;
}
if (tmpAry[dictTitle[LabelTag]] != "N/A")
{
double intensity = Convert.ToDouble(tmpAry[dictTitle[LabelTag]]);
if (intensity < 0)
{
intensity = 0;
}
dictData[GlycanKey].Add(LabelTag, intensity);
}
}
}
sr.Close();
#endregion
#region Generate Quant Images
//foreach(string Gkey in dictData.Keys)
//ZedGraph.ZedGraphControl zgcGlycan = null;
Parallel.ForEach(dictData.Keys, new ParallelOptions()
{
MaxDegreeOfParallelism = MaxDegreeParallelism
}, Gkey =>
{
//ZedGraphControl zgcGlycan = null;
try
{
Chart cht = new Chart();
cht.Size = new Size(2400, 1200);
cht.ChartAreas.Add("Default");
cht.ChartAreas["Default"].AxisX.Title = "Labeling";
cht.ChartAreas["Default"].AxisX.TitleFont = new Font("Arial", 24, FontStyle.Bold);
cht.ChartAreas["Default"].AxisX.LabelStyle.Format = "{F2}";
cht.ChartAreas["Default"].AxisX.LabelStyle.Font = new Font("Arial", 24, FontStyle.Bold);
cht.ChartAreas["Default"].AxisX.MajorGrid.LineDashStyle = ChartDashStyle.Dash;
cht.ChartAreas["Default"].AxisY.Title = "Abundance(%)";
cht.ChartAreas["Default"].AxisY.TitleFont = new Font("Arial", 24, FontStyle.Bold);
cht.ChartAreas["Default"].AxisY.LabelStyle.Format = "{0.#E+00}";
cht.ChartAreas["Default"].AxisY.LabelStyle.Font = new Font("Arial", 24, FontStyle.Bold);
cht.ChartAreas["Default"].AxisY.MajorGrid.LineDashStyle = ChartDashStyle.Dash;
cht.Titles.Add("Default");
cht.Titles[0].Text = "Glycan: " + Gkey;
cht.Titles[0].Font = new Font("Arial", 24, FontStyle.Bold);
cht.Legends.Add("Default");
cht.Legends["Default"].Docking = Docking.Bottom;
cht.Legends["Default"].Alignment = StringAlignment.Center;
cht.Legends["Default"].LegendStyle = LegendStyle.Row;
cht.Legends["Default"].Font = new Font("Arial", 24, FontStyle.Bold);
Dictionary <enumLabelingTag, double> dictLabelIntensity = new Dictionary <enumLabelingTag, double>();
double YMax = dictData[Gkey].Values.Max();
List <string> labels = new List <string>();
List <LCPointPair> ppl = new List <LCPointPair>();
int i = 0;
foreach (string labelTag in lstLabelingTag)
{
labels.Add(labelTag);
if (!dictTitle.ContainsKey(labelTag) || !dictData[Gkey].ContainsKey(labelTag))
{
ppl.Add(new LCPointPair(i, 0));
}
else
{
ppl.Add(new LCPointPair(i, dictData[Gkey][labelTag] / YMax * 100));
}
i++;
}
Series myBar = cht.Series.Add("Data");
myBar.ChartType = SeriesChartType.Bar;
for (int j = 0; j < ppl.Count; j++)
{
myBar.Points.AddY(ppl[j].Intensity);
myBar.Points[j].AxisLabel = lstLabelingTag[j];
}
cht.SaveImage(Dir + "\\Quant-" + Gkey + ".png", ChartImageFormat.Png);
}
catch (Exception ex)
{
throw ex;
}
#endregion
});
}
public static void GenQuantImg(string argQuantFile, enumGlycanLabelingMethod argLabelingMethod,
string argExportFolder)
{
string Dir = argExportFolder + "\\Pic";
if (!Directory.Exists(Dir))
{
Directory.CreateDirectory(Dir);
}
List<Color> LstColor = new List<Color>()
{
Color.DarkCyan,
Color.DarkGoldenrod,
Color.DarkGray,
Color.DarkGreen,
Color.DarkKhaki,
Color.DarkMagenta,
Color.DarkOliveGreen,
Color.DarkOrchid,
Color.DarkRed,
Color.DarkSalmon,
Color.DarkSeaGreen,
Color.DarkSlateBlue,
Color.DarkSlateGray,
Color.DarkTurquoise,
Color.DarkViolet,
Color.DeepPink,
Color.DeepSkyBlue
};
//Get Title
Dictionary<string, int> dictTitle = new Dictionary<string, int>();
StreamReader sr = new StreamReader(argQuantFile);
string tmp = "";
bool isLabeling = false;
tmp = sr.ReadLine();
#region Read Title
string[] tmpAry = tmp.Split(',');
string LabelTitle = "";
dictTitle.Add("Glycan", 0);
List<string> lstLabelingTag = new List<string>();
if (argLabelingMethod == enumGlycanLabelingMethod.DRAG)
{
lstLabelingTag.Add("DRAG_Light(Adjusted 1)");
lstLabelingTag.Add("DRAG_Heavy(Adjusted 1)");
}
else if (argLabelingMethod == enumGlycanLabelingMethod.MultiplexPermethylated)
{
lstLabelingTag.Add("MP_CH3");
lstLabelingTag.Add("MP_CH2D");
lstLabelingTag.Add("MP_CHD2");
lstLabelingTag.Add("MP_CD3");
lstLabelingTag.Add("MP_13CH3");
lstLabelingTag.Add("MP_13CHD2");
lstLabelingTag.Add("MP_13CD3");
}
for (int i = 1; i < tmpAry.Length; i++)
{
if (argLabelingMethod == enumGlycanLabelingMethod.MultiplexPermethylated &&
tmpAry[i].StartsWith("MP"))
{
dictTitle.Add(tmpAry[i], i + 3);
// MP_CH3 Normalization Factor Estimated Purity Normalizted and Adjusted Abundance (Adjusted Factor=1)
}
else if (argLabelingMethod == enumGlycanLabelingMethod.DRAG &&
tmpAry[i].Contains("DRAG_Light(Adjusted 1)") ||
tmpAry[i].Contains("DRAG_Heavy(Adjusted 1)"))
{
dictTitle.Add(tmpAry[i], i);
}
}
#endregion
#region Get Data
Dictionary<string, Dictionary<string, double>> dictData =
new Dictionary<string, Dictionary<string, double>>();
while(!sr.EndOfStream)
{
tmp = sr.ReadLine();
tmpAry = tmp.Split(',');
string GlycanKey = tmpAry[dictTitle["Glycan"]];
if (!dictData.ContainsKey(GlycanKey))
{
dictData.Add(GlycanKey, new Dictionary<string, double>());
}
foreach (string LabelTag in lstLabelingTag)
{
if (!dictTitle.ContainsKey(LabelTag))
{
continue;
}
if (tmpAry[dictTitle[LabelTag]] != "N/A")
{
double intensity = Convert.ToDouble(tmpAry[dictTitle[LabelTag]]);
if (intensity < 0)
{
intensity = 0;
}
dictData[GlycanKey].Add(LabelTag, intensity);
}
}
}
sr.Close();
#endregion
#region Generate Quant Images
//foreach(string Gkey in dictData.Keys)
//ZedGraph.ZedGraphControl zgcGlycan = null;
Parallel.ForEach(dictData.Keys, new ParallelOptions() { MaxDegreeOfParallelism = MaxDegreeParallelism }, Gkey =>
{
//ZedGraphControl zgcGlycan = null;
try
{
Chart cht = new Chart();
cht.Size = new Size(2400, 1200);
cht.ChartAreas.Add("Default");
cht.ChartAreas["Default"].AxisX.Title = "Labeling";
cht.ChartAreas["Default"].AxisX.TitleFont = new Font("Arial", 24, FontStyle.Bold);
cht.ChartAreas["Default"].AxisX.LabelStyle.Format = "{F2}";
cht.ChartAreas["Default"].AxisX.LabelStyle.Font = new Font("Arial", 24, FontStyle.Bold);
cht.ChartAreas["Default"].AxisX.MajorGrid.LineDashStyle = ChartDashStyle.Dash;
cht.ChartAreas["Default"].AxisY.Title = "Abundance(%)";
cht.ChartAreas["Default"].AxisY.TitleFont = new Font("Arial", 24, FontStyle.Bold);
cht.ChartAreas["Default"].AxisY.LabelStyle.Format = "{0.#E+00}";
cht.ChartAreas["Default"].AxisY.LabelStyle.Font = new Font("Arial", 24, FontStyle.Bold);
cht.ChartAreas["Default"].AxisY.MajorGrid.LineDashStyle = ChartDashStyle.Dash;
cht.Titles.Add("Default");
cht.Titles[0].Text = "Glycan: " + Gkey;
cht.Titles[0].Font = new Font("Arial", 24, FontStyle.Bold);
cht.Legends.Add("Default");
cht.Legends["Default"].Docking = Docking.Bottom;
cht.Legends["Default"].Alignment = StringAlignment.Center;
cht.Legends["Default"].LegendStyle = LegendStyle.Row;
cht.Legends["Default"].Font = new Font("Arial", 24, FontStyle.Bold);
Dictionary<enumLabelingTag, double> dictLabelIntensity = new Dictionary<enumLabelingTag, double>();
double YMax = dictData[Gkey].Values.Max();
List<string> labels = new List<string>();
List<LCPointPair> ppl = new List<LCPointPair>();
int i = 0;
foreach (string labelTag in lstLabelingTag)
{
labels.Add(labelTag);
if (!dictTitle.ContainsKey(labelTag) || !dictData[Gkey].ContainsKey(labelTag))
{
ppl.Add(new LCPointPair(i, 0));
}
else
{
ppl.Add(new LCPointPair(i, dictData[Gkey][labelTag] / YMax * 100));
}
i++;
}
Series myBar = cht.Series.Add("Data");
myBar.ChartType = SeriesChartType.Bar;
for(int j =0; j<ppl.Count;j++)
{
myBar.Points.AddY(ppl[j].Intensity);
myBar.Points[j].AxisLabel = lstLabelingTag[j];
}
cht.SaveImage(Dir + "\\Quant-" + Gkey + ".png",ChartImageFormat.Png);
}
catch (Exception ex)
{
throw ex;
}
#endregion
});
}
